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Titolo:
UNDERSTANDING THE DENDRITIC CELL LINEAGE THROUGH A STUDY OF CYTOKINE RECEPTORS
Autore:
KAMPGEN E; KOCH F; HEUFLER C; EGGERT A; GILL LL; GILLIS S; DOWER SK; ROMANI N; SCHULER G;
Indirizzi:
INNSBRUCK UNIV,DEPT DERMATOL,ANICHSTR 35 A-6020 INNSBRUCK AUSTRIA INNSBRUCK UNIV,DEPT DERMATOL A-6020 INNSBRUCK AUSTRIA UNIV WURZBURG,DEPT DERMATOL W-8700 WURZBURG GERMANY BASEL INST IMMUNOL BASEL SWITZERLAND IMMUNEX CORP SEATTLE WA 00000
Titolo Testata:
The Journal of experimental medicine
fascicolo: 6, volume: 179, anno: 1994,
pagine: 1767 - 1776
SICI:
0022-1007(1994)179:6<1767:UTDCLT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; EPIDERMAL LANGERHANS CELLS; TUMOR-NECROSIS-FACTOR; MOUSE BONE-MARROW; HIGH-AFFINITY RECEPTOR; IL-1 RECEPTOR; SURFACE PHENOTYPE; IMMUNE-RESPONSES; GENE-EXPRESSION; UP-REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
E. Kampgen et al., "UNDERSTANDING THE DENDRITIC CELL LINEAGE THROUGH A STUDY OF CYTOKINE RECEPTORS", The Journal of experimental medicine, 179(6), 1994, pp. 1767-1776

Abstract

Dendritic cells form a system of antigen presenting cells that are specialized to stimulate T lymphocytes, including quiescent T cells. Thelineage of dendritic cells is not fully characterized, although priorstudies have shown that growth and differentiation are controlled by cytokines, particularly granulocyte/macrophage colony-stimulating factor (GM-CSF). To further elucidate the nature and control of the dendritic cell lineage, we have studied the expression of specific cytokine receptors. Sufficient numbers of dendritic cells were purified from spleen and skin to do quantitative binding studies with radiolabeled M-CSF, GM-CSF, and interleukin 1 (IL-1). To verify the nonlymphoid natureof dendritic cells, we made an initial search for rearrangements in Tcell receptor and immunoglobulin genes and none were found. M-CSF binding sites, a property of mononuclear phagocytes, also were absent. Incontrast, GM-CSF receptors were abundant on mature dendritic cells, with similar to 3,000 binding sites/cell with a single K-d of 500-1,000pM. Substantial numbers of high affinity (<100 pM) IL-1 binding siteswere identified as well; cultured epidermal dendritic cells (i.e., epidermal Langerhans cells) had 500/cell and spleen dendritic cells similar to 70/cell. Cross-linking approaches showed the 80-kD species thatis expected of high-affinity type 1 IL-1 receptor. Anti-type 1 IL-1 receptor (R) mAbs also visualized these receptors by how cytometry on freshly isolated epidermal dendritic cells. These results provide new evidence that dendritic cells represent a differentiation pathway distinct from lymphocytes and monocytes. Together with recent findings on the effects of IL-1 and GM-CSF on epidermal dendritic cells in situ (see Results and Discussion), the data lead to a Proposal whereby IL-1 signals IL-1R to upregulate GM-CSF receptors and thereby, the observed responsiveness of dendritic cells to GM-CSF for growth, viability, and function.

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Documento generato il 27/11/20 alle ore 22:35:18