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Titolo:
OPTIMAL CONDITIONS OF 1 M NACL SPLITTING TECHNIQUE TO DEMONSTRATE BASEMENT-MEMBRANE ZONE ANTIGENS IN BULLOUS PEMPHIGOID, EPIDERMOLYSIS-BULLOSA ACQUISITA AND LINEAR IGA BULLOUS DERMATOSES
Autore:
JENKINS RE; RODENAS J; BHOGAL BS; BLACK MM;
Indirizzi:
UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,ST THOMAS HOSP,ST JOHNS INST DERMATOL LONDON SE1 7EH ENGLAND
Titolo Testata:
Dermatology
, volume: 189, anno: 1994, supplemento:, 1
pagine: 133 - 134
SICI:
1018-8665(1994)189:<133:OCO1MN>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Keywords:
BULLOUS PEMPHIGOID; LINEAR IGA BULLOUS DERMATOSES; EPIDERMOLYSIS BULLOSA ACQUISITA; BASEMENT MEMBRANE ZONE ANTIBODIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
2
Recensione:
Indirizzi per estratti:
Citazione:
R.E. Jenkins et al., "OPTIMAL CONDITIONS OF 1 M NACL SPLITTING TECHNIQUE TO DEMONSTRATE BASEMENT-MEMBRANE ZONE ANTIGENS IN BULLOUS PEMPHIGOID, EPIDERMOLYSIS-BULLOSA ACQUISITA AND LINEAR IGA BULLOUS DERMATOSES", Dermatology, 189, 1994, pp. 133-134

Abstract

We compare a series of conditions for inducing lamina lucida separation through normal skin specimens using 1 M NaCl for the detection of basement membrane zone auto-antibodies using indirect immunofluorescence. Skin was split at room temperature and at 4 degrees C with and without phenylmethylsulphonyl fluoride. Our results demonstrate that for the detection of bullous pemphigoid and epidermal-binding linear IgA bullous dermatoses antibodies skin should be split at 4 degrees C with enzyme inhibitors. For epidermolysis bullosa acquisita and dermal-binding antibodies skin can be split at room temperature.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 11:35:00