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Titolo:
MECHANISM OF ANTIBODY-DEPENDENT NATURAL-KILLER CELL-MEDIATED AK-5 TUMOR-CELL DEATH
Autore:
KAUSALYA S; HEGDE SP; BRIGHT JJ; KHAR A;
Indirizzi:
CTR CELLULAR & MOLEC BIOL,UPPAL RD HYDERABAD 500007 INDIA CTR CELLULAR & MOLEC BIOL HYDERABAD 500007 INDIA
Titolo Testata:
Experimental cell research
fascicolo: 2, volume: 212, anno: 1994,
pagine: 285 - 290
SICI:
0014-4827(1994)212:2<285:MOANCA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUS IMMUNOLOGICAL REGRESSION; DNA FRAGMENTATION; NK CELLS; PERFORIN; HISTIOCYTOMA; EXPRESSION; CYTOLYSIS; LYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
S. Kausalya et al., "MECHANISM OF ANTIBODY-DEPENDENT NATURAL-KILLER CELL-MEDIATED AK-5 TUMOR-CELL DEATH", Experimental cell research, 212(2), 1994, pp. 285-290

Abstract

The mechanism of antibody-dependent natural killer (NK) cell-mediatedkilling of a histiocytic tumor, AK-5, was studied. In vitro exposure of immune NK cells with antibody-primed AK-5 cells induced the formation of stable NK-AK-5 conjugates. The frequency of conjugate formation after 1 h coculture of immune NK cells was severalfold higher than that of normal cells. The occurrence of conjugate formation and the disintegration of conjugated targets only in the presence of anti-AK-5 antibody revealed the antibody dependence in recognition, conjugate formation, and triggering of lytic machinery leading to AK-5 cell death. Coculture of immune NK cells with AK-5 cells in the presence of anti-AK-5antibody induced the expression of perforin mRNA as well as accumulation of protein in the NK cells and extensive fragmentation of AK-5 cell DNA, thereby indicating the involvement of necrosis and apoptosis inthe AK-5 cell death. The antibody-dependent expression of perforin aswell as DNA fragmentation indicated these events to be consequent to E-T conjugate formation. The observation of target cell DNA fragmentation revealed that the internal target disintegration is the first event which is followed by membrane damage leading to efficient tumor destruction by the host immune system. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:35:41