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Titolo:
EVIDENCE FOR A GLUCOSE 1-PHOSPHATE TRANSLOCATOR IN STORAGE TISSUE AMYLOPLASTS OF POTATO (SOLANUM-TUBEROSUM) SUSPENSION-CULTURED CELLS
Autore:
KOSEGARTEN H; MENGEL K;
Indirizzi:
INST PLANT NUTR,SUDANLAGE 6 D-35390 GIESSEN GERMANY INST PLANT NUTR D-35390 GIESSEN GERMANY
Titolo Testata:
Physiologia Plantarum
fascicolo: 1, volume: 91, anno: 1994,
pagine: 111 - 120
SICI:
0031-9317(1994)91:1<111:EFAG1T>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACER-PSEUDOPLATANUS L; STARCH SYNTHESIS; CARBOHYDRATE-METABOLISM; TRIOSE PHOSPHATES; MAIZE ENDOSPERM; INNER MEMBRANE; TRANSPORT; ENVELOPE; BIOSYNTHESIS; PLASTIDS;
Keywords:
AMYLOPLAST; GLUCOSE 1-PHOSPHATE; GLUCOSE 1-PHOSPHATE TRANSLOCATOR; POTATO; SOLANUM TUBEROSUM; STARCH SYNTHESIS; STORAGE TISSUE; TRIOSE PHOSPHATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
H. Kosegarten e K. Mengel, "EVIDENCE FOR A GLUCOSE 1-PHOSPHATE TRANSLOCATOR IN STORAGE TISSUE AMYLOPLASTS OF POTATO (SOLANUM-TUBEROSUM) SUSPENSION-CULTURED CELLS", Physiologia Plantarum, 91(1), 1994, pp. 111-120

Abstract

Transport of glucose l-phosphate (GIP) and highly purified triose phosphate into storage tissue amyloplasts was studied. Isolated amyloplasts from potato (Solanum tuberosum L., dihaploid stock, HH 258) were transport-functional and metabolically active in starch synthesis. Fourty percent of the amyloplasts were intact and there was only a small degree (0-1.6%) of contamination by other cellular compartments. G1P showed a clear uptake pattern paralleled by starch synthesis. Uptake of triose phosphates was virtually nil. Uptake of G1P was pH dependent with a sharp maximum at pH 5.7 and showed Michaelis-Menten kinetics with an apparent K-m of 0.5 mM. Temperature influenced the rate of uptake, the highest rate being al 25 degrees C. Fructose I-phosphate, ADP-glucose, glucose, and inorganic phosphate inhibited the uptake of GIP. Uptake was also inhibited by DIDS (1-25 mu M) and by Phloretin (45-750 muM) It is therefore concluded that the transport of G1P across the inner amyloplast membrane is mediated by a hexose phosphate translocator selective for phosphate and glucose moieties of the molecule. Considering the low pH maximum for GIP uptake it is possible that the uptake of G1P, and eventually starch synthesis, is regulated by an acidification of the intermembrane space by proton pumps of the inner amyloplast membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:04:12