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Titolo:
LUMINARIN-3 AS A DERIVATIZATION REAGENT FOR THE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CYTIDINE, ADENOSINE AND RELATED NUCLEOTIDES WITH FLUOROMETRIC DETECTION
Autore:
TRAORE F; FENTE C; PROGNON P; MAHUZIER G;
Indirizzi:
UPS,CEP,CHIM ANALYT LAB 2 F-92290 CHATENAY MALABRY FRANCE UNIV CONAKRY,FAC MED & PHARM CONAKY GUINEA FAC VET LUGO,DEPT QUIM ANAL NUTR & BROMATOL E-27002 LUGO SPAIN
Titolo Testata:
Analytica chimica acta
fascicolo: 1-2, volume: 290, anno: 1994,
pagine: 94 - 102
SICI:
0003-2670(1994)290:1-2<94:LAADRF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENINE-NUCLEOTIDES; FLUORESCENCE DETECTION; HUMAN-PLASMA; QUANTITATION; HYPOXANTHINE; HYDRAZIDE; SAMPLES; INOSINE; BIOTIN; ASSAY;
Keywords:
LIQUID CHROMATOGRAPHY; FLUOROMETRY; LUMINARIN 3; NUCLEOSIDES; NUCLEOTIDES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
F. Traore et al., "LUMINARIN-3 AS A DERIVATIZATION REAGENT FOR THE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CYTIDINE, ADENOSINE AND RELATED NUCLEOTIDES WITH FLUOROMETRIC DETECTION", Analytica chimica acta, 290(1-2), 1994, pp. 94-102

Abstract

Luminarin 3 is a quinolizinocoumarine labelling reagent for carbonyl compounds, reacting via its hydrazide function. It was found to be a highly sensitive fluorescence reactive probe for nucleosides and nucleotides in liquid chromatography. Luminarin 3 reacts with the dialdehydeinduced by preliminary periodate oxidation of the ribose moiety of nucleosides and nucleotides in 100 mM acetate buffer (at pH 3.0 or 4.0, depending on the derivative), to give highly fluorescent morpholino derivatives. The luminarin 3 derivatives of cytidine (Cyd), adenosine (Ado) and corresponding nucleotides were separated by chromatography on a Spherisorb cyano 5-mu m column (300 x 4.6 mm i.d.), with a mixture of methanol and 100 mM phosphate buffer pH 6.0 (30/70, v/v), followed by fluorescence detection. In such a way the separation can easily be optimized for the eight nucleotides by variation of the methanol content of the mobile phase. Typical detection limits (in amount injected with signal/noise ratio of 3) were 40 fmol for Cyd; 52 fmol for CMP; 71 fmol for CDP; 36 fmol for CTP; 667 fmol for Ado, 70 fmol for AMP, 28 fmol for ADP and 39 fmol for ATP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:35:51