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Titolo:
IDENTIFICATION OF THE U-937 MEMBRANE-ASSOCIATED PROTEINASE INTERACTING WITH THE V3 LOOP OF HIV-1 GP120 AS CATHEPSIN-G
Autore:
AVRIL LE; DIMARTINOFERRER M; PIGNEDE G; SEMAN M; GAUTHIER F;
Indirizzi:
UNIV TOURS,FAC MED,ENZYMOL & CHIM PROT LAB,CNRS,URA 1334,2 BLVD TONNELLE F-37032 TOURS FRANCE UNIV TOURS,FAC MED,ENZYMOL & CHIM PROT LAB,CNRS,URA 1334 F-37032 TOURS FRANCE UNIV DENIS DIDEROT,IMMUNODIFFERENCIAT LAB PARIS FRANCE
Titolo Testata:
FEBS letters
fascicolo: 1, volume: 345, anno: 1994,
pagine: 81 - 86
SICI:
0014-5793(1994)345:1<81:IOTUMP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL PLASMA-MEMBRANES; ENVELOPE GLYCOPROTEIN; HUMAN LYMPHOCYTES-T4+; PROTEOLYTIC CLEAVAGE; SOLUBLE CD4; INFECTION; U937; MONOCYTES; SURFACE; DOMAIN;
Keywords:
HIV-1; GP120 V3 LOOP; PROTEINASE; CATHEPSIN G;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
L.E. Avril et al., "IDENTIFICATION OF THE U-937 MEMBRANE-ASSOCIATED PROTEINASE INTERACTING WITH THE V3 LOOP OF HIV-1 GP120 AS CATHEPSIN-G", FEBS letters, 345(1), 1994, pp. 81-86

Abstract

We have purified a serine proteinase from the membrane of U-937 cellsthat was inhibited in a tight-binding manner by recombinant gp120 andby peptides mimicking the V3 loop of gp120 [(1993) FEBS Lett. 317, 167-172]. This proteinase has now been characterized, both structurally and functionally. It has a dual trypsin- and chymotrypsin-like specificity, and N-terminal sequence analysis of the first 32 residues indicates complete identity with leukocyte cathepsin G. Cathepsin G-like material was located at the surface of U-937 cells using a monoclonal antibody directed against leukocyte cathepsin G, and polyclonal anti-cathepsin C antibodies precipitated the purified proteinase. However, the U-937 enzyme differs slightly from commercial leukocyte cathepsin G inits apparent M, because of different glycosylation. No other protein structurally related to cathepsin G was found upon screening a U-937 cDNA library using several oligonucleotide probes constructed from the membrane proteinase N-terminal amino acid sequence. The possible interaction of a cathepsin G-like proteinase at the surface of U-937 cells with the V3 loop of HIV-1 gp120 is discussed.

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Documento generato il 04/12/20 alle ore 19:37:23