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Titolo:
FURTHER EVIDENCE FOR AN ENDOGENOUS DIGITALIS-LIKE COMPOUND IN NEWBORNAND ADULT PLASMA DETECTED BY ANTI-OUABAIN ANTISERUM
Autore:
BALZAN S; MONTALI U; DIBARTOLO V; GHIONE S;
Indirizzi:
UNIV PISA,CNR,INST CLIN PHYSIOL,VIA SAVI 8 I-56126 PISA ITALY UNIV PISA,INST BIOL CHEM I-56126 PISA ITALY CNR,INST MUTAGENESIS & DIFFERENTIAT PISA PISA ITALY
Titolo Testata:
Life sciences
fascicolo: 12, volume: 60, anno: 1997,
pagine: 893 - 898
SICI:
0024-3205(1997)60:12<893:FEFAED>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIGOXIN-LIKE IMMUNOREACTIVITY; BOVINE HYPOTHALAMUS; STRUCTURAL-ANALYSIS; CORD BLOOD; CHROMATOGRAPHY; PURIFICATION; INHIBITION; EXTRACTS; ATPASE; SERUM;
Keywords:
ENDOGENOUS DIGITALIS-LIKE FACTOR; HUMAN NEWBORN PLASMA; OUABAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
S. Balzan et al., "FURTHER EVIDENCE FOR AN ENDOGENOUS DIGITALIS-LIKE COMPOUND IN NEWBORNAND ADULT PLASMA DETECTED BY ANTI-OUABAIN ANTISERUM", Life sciences, 60(12), 1997, pp. 893-898

Abstract

It is widely but not unanimously accepted that one or more endogenousdigitalis-like factors (EDLF) circulate in human plasma. In this paper we provide confirmatory evidence that newborn plasma contains a factor with immunological and biological properties similar to ouabain anddemonstrate that this factor may be present also in the adult. In fact, we obtained in newborn and adult plasma extracts, identical HPLC elution profiles of ouabain-like immunoreactivity and Rb-86 erythrocyte uptake inhibitory activity with a major peak corresponding to the retention time of ouabain. The fact that immunoreactivity and biological digitalis-like activity in the peak are due to an identical substance is strongly supported by the correlation between RIA and Rb-86 uptake inhibitory values observed in the purified fractions. Finally, the strong correlation between immunoreactivity observed in plasma samples after simple SepPak C-18 extraction and after additional HPLC suggests that less purified samples may be assayed for screening purposes. However, for a more quantitative assessment, this simple extraction method needs a subsequent HPLC purification for eliminating an overestimation of values due to cross-reacting impurities.

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Documento generato il 27/11/20 alle ore 21:56:29