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Titolo:
LOCALIZATION OF HISTONE-LIKE PROTEINS IN THERMOPHILIC ARCHAEA BY IMMUNOGOLD ELECTRON-MICROSCOPY
Autore:
BOHRMANN B; KELLENBERGER E; ARNOLDSCHULZGAHMEN B; SREENIVAS K; SURYANARAYANA T; STROUP D; REEVE JN;
Indirizzi:
HOFFMANN LA ROCHE AG,PHARMA RES NEW TECHNOL,DEPT PHYS,GRENZACHERSTR 124-B65-R414 CH-4002 BASEL SWITZERLAND UNIV BASEL,BIOZENTRUM,DEPT MICROBIOL CH-4056 BASEL SWITZERLAND UNIV DORTMUND,INST ARBEITSPHYSIOL D-44139 DORTMUND GERMANY UNIV HYDERABAD,DEPT BIOCHEM HYDERABAD 500134 ANDHRA PRADESH INDIA OHIO STATE UNIV,DEPT MICROBIOL COLUMBUS OH 43210
Titolo Testata:
Journal of structural biology
fascicolo: 1, volume: 112, anno: 1994,
pagine: 70 - 78
SICI:
1047-8477(1994)112:1<70:LOHPIT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARCHAEBACTERIUM SULFOLOBUS-ACIDOCALDARIUS; DNA-BINDING PROTEINS; METHANOTHERMUS-FERVIDUS; ULTRASTRUCTURAL-LOCALIZATION; BACTERIA; SHAPE; HMF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
B. Bohrmann et al., "LOCALIZATION OF HISTONE-LIKE PROTEINS IN THERMOPHILIC ARCHAEA BY IMMUNOGOLD ELECTRON-MICROSCOPY", Journal of structural biology, 112(1), 1994, pp. 70-78

Abstract

Immunogold staining combined with heavy metal-based DNA immunostaining of ultrathin sections of cryofixed, freeze-substituted, and resin-embedded cells has revealed that in the hyperthermophilic archaeon, Sulfolobus acidocaldarius, the histone-like proteins HSNP-A and HSNP-C' are colocalized with DNA in the ribosome-free nucleoid, whereas DBNP-B is located exclusively in the ribosome-containing cytoplasm. Following chemical fixation and solvent dehydration the chromatin was resistant to aggregation, indicative of a high protein content. Immunogold labeling of chromatin released from isolated nucleoids of S. acidocaldariusdemonstrated that the helix-stabilizing protein HSNP-C' was randomly distributed on both filamentous DNA and on more globular material present within distinct nucleoid subdomains. DNA-dependent RNA polymerase molecules were located by immunogold staining predominantly in the cytoplasmic region immediately surrounding the nucleoid in S. acidocaldarius, presumably at the locations of active transcription. Affinity-purified antibodies raised against HMf, a histone-like protein isolated from the hyperthermophilic and only distantly related methanogenic archaeon Methanothermus fervidus, were also found to be localized almost exclusively to the DNA-containing, ribosome-free nucleoid region. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 11:18:47