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Titolo:
SUBCELLULAR CHARACTERIZATION OF GLYCOPROTEINS IN THE PRINCIPAL CELLS OF HUMAN GALLBLADDER - A LECTIN CYTOCHEMICAL STUDY
Autore:
MADRID JF; CASTELLS MT; MARTINEZMENARGUEZ JA; AVILES M; HERNANDEZ F; BALLESTA J;
Indirizzi:
UNIV MURCIA,SCH MED,DEPT CELL BIOL,HISTOL & GEN EMBRYOL SECT E-30071 MURCIA SPAIN UNIV MURCIA,SCH MED,DEPT CELL BIOL,HISTOL & GEN EMBRYOL SECT E-30071 MURCIA SPAIN UNIV BASQUE COUNTRY,SCH MED & DENT,DEPT CELL BIOL & MORPHOL SCI VIZCAYA SPAIN
Titolo Testata:
Histochemistry
fascicolo: 3, volume: 101, anno: 1994,
pagine: 195 - 204
SICI:
0301-5564(1994)101:3<195:SCOGIT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACETYL-D-GALACTOSAMINE; ELECTRON-MICROSCOPIC DEMONSTRATION; RICINUS-COMMUNIS AGGLUTININ; SIALIC-ACID RESIDUES; BINDING-PROPERTIES; GOLD COMPLEXES; LOTUS-TETRAGONOLOBUS; MAACKIA-AMURENSIS; OLIGOSACCHARIDES; MUCIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
J.F. Madrid et al., "SUBCELLULAR CHARACTERIZATION OF GLYCOPROTEINS IN THE PRINCIPAL CELLS OF HUMAN GALLBLADDER - A LECTIN CYTOCHEMICAL STUDY", Histochemistry, 101(3), 1994, pp. 195-204

Abstract

Gallbladder mucus is mainly composed of glycoproteins, which seem to play a critical role in cholesterol nucleation during gallstone formation. The biosynthetic pathway and sequential processing as well as thecharacterization of the oligosaccharide sidechains of human gallbladder secretory glycoproteins have not been completely defined. The aim of the present study is the subcellular characterization of the glycoproteins in the principal cells of human gallbladder. Principal cells ofnormal human gallbladder were studied by means of a variety of cytochemical techniques, including lectin histochemistry, enzyme and chemical treatments, immunocytochemistry and lectin-gold technology. Fucose, galactose, N-acetylglucosamine, N-acetylgalactosamine and N-acetylneuraminic acid residues were detected in mucous granules, Golgi apparatusand apical membrane of principal cells. Mannose residues were only observed in dense bodies. Oligosaccharide side-chains of the glycoproteins contained in the biliary mucus are synthesized in the Golgi apparatus of the principal cells of the gallbladder epithelium and are also contained in the mucous granules of these cells. Terminal N-acetylneuraminic acid(alpha 2-3)galactose(beta 1-3)N-acetylgalactosamine, N-acetylneuraminic acid(alpha 2-3)galactose(beta 1-4)N-acetylglucosamine and galactose(beta 1-4)N-acetylglucosamine sequences are contained in the oligosaccharide chains of gallbladder mucus glycoproteins. The dense bodies detected in the cytoplasm of the principal cells contained N-linked glycoproteins. Mucin-type O-linked glycoproteins were the main components of the mucous granules although some N-linked chains were alsodetected.

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Documento generato il 07/07/20 alle ore 06:20:35