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Titolo:
CELLULAR UPTAKE OF OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATES AND THEIR ANALOGS
Autore:
TEMSAMANI J; KUBERT M; TANG JY; PADMAPRIYA A; AGRAWAL S;
Indirizzi:
HYBRIDON INC,1 INNOVAT DR WORCESTER MA 01605 HYBRIDON INC,1 INNOVAT DR WORCESTER MA 01605
Titolo Testata:
Antisense research and development
fascicolo: 1, volume: 4, anno: 1994,
pagine: 35 - 42
SICI:
1050-5261(1994)4:1<35:CUOOPA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; CHRONICALLY INFECTED-CELLS; ANTISENSE OLIGONUCLEOTIDES; PHARMACOKINETICS; INVITRO; INHIBITION; STABILITY; MICE; RNA; OLIGODEOXYRIBONUCLEOTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J. Temsamani et al., "CELLULAR UPTAKE OF OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATES AND THEIR ANALOGS", Antisense research and development, 4(1), 1994, pp. 35-42

Abstract

The uptake and intracellular distribution of oligonucleotide phosphorothioates (S-oligonucleotides) and their analogs by various cells lines were studied using internally S-35-labeled oligonucleotides. Intracellular accumulation starts in the first 2 hr and reaches a maximum at about 16 hr. A decrease of intracellular concentration of the S-oligonucleotide was observed after 16 hr, probably due to a significant efflux transport. Cellular uptake was dependent on the extracellular concentration. The intracellular concentration was significantly higher than that in the medium. The uptake and the intracellular distribution were different in the various cell tines studied. Comparative studies ofthe uptake of the S-oligonucleotide and various 3' end-modified S-oligonucleotides show that end modified S-oligonucleotides with a hydrophobic group significantly increases the uptake. The introduction of methylphosphonothioate linkages at the 3' end of the S-oligonucleotide also resulted in an increase in uptake.

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Documento generato il 22/09/20 alle ore 07:41:38