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Titolo:
LONG-STANDING PROTECTION OF MACAQUES AGAINST CELL-FREE HIV-2 WITH A HIV-2 ISCOM VACCINE
Autore:
PUTKONEN P; BJORLING E; AKERBLOM L; THORSTENSSON R; LOVGREN K; BENTHIN L; CHIODI F; MOREIN B; BIBERFELD G; NORRBY E; WIGZELL H;
Indirizzi:
KAROLINSKA INST,SWEDISH INST INFECT DIS CONTROL,DEPT IMMUNOL S-10521 STOCKHOLM SWEDEN KAROLINSKA INST,SWEDISH INST INFECT DIS CONTROL,DEPT VIROL STOCKHOLM SWEDEN KAROLINSKA INST,CTR MICROBIOL & TUMOR BIOL STOCKHOLM SWEDEN NATL VET INST,BIOMED CENTRUM S-75123 UPPSALA SWEDEN
Titolo Testata:
Journal of acquired immune deficiency syndromes
fascicolo: 6, volume: 7, anno: 1994,
pagine: 551 - 559
SICI:
0894-9255(1994)7:6<551:LPOMAC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; CYNOMOLGUS MONKEYS; SYNTHETIC PEPTIDES; SIV INFECTION; CHIMPANZEES; ANTIBODY; ENVELOPE; NEUTRALIZATION; IMMUNIZATION; GLYCOPROTEIN;
Keywords:
HIV VACCINE; HIV-2; ANIMAL MODEL; ISCOMS; CYNOMOLGUS MONKEYS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
P. Putkonen et al., "LONG-STANDING PROTECTION OF MACAQUES AGAINST CELL-FREE HIV-2 WITH A HIV-2 ISCOM VACCINE", Journal of acquired immune deficiency syndromes, 7(6), 1994, pp. 551-559

Abstract

We investigated the capacity of two immunostimulating-complex (iscom)formulations including inactivated native HIV-2 viral proteins and selected peptides to induce protective immunity against HIV-2 in a nonhuman primate. Four cynomolgus monkeys were first immunized with five i.m. injections of purified detergent-disrupted HIV-2 virions (total dose, 0.7 mg) in iscoms over a period of 16 months. At months 18 and 20, all four macaques were given booster immunizations with iscom-coupled V3-derived synthetic peptides representing a dominating neutralizing region of HIV-2 gp125. Two weeks after the final dose of vaccine, the four vaccinated animals, together with four controls, were challenged i.v. with 10 monkey infectious doses (MID(50)) of monkey-cell-grown homologous cell-free virus, HIV-2(SBL-6669/H5). After the challenge, the four control animals became readily infected; however, three of four vaccinated animals were protected as shown by repeated negative virus isolations and negative polymerase chain reaction for viral DNA and by failure to transmit HIV-2 infection with whole blood and lymph node cells into naive cynomolgus macaques. One of three protected animals showed an anamnestic antibody response to a dominating antigenic site, indicating possible limited virus replication. The vaccine-protected monkeys were subsequently resistant to rechallenge infection at 12, 15, and 18 months after the first challenge, suggesting that a reasonable duration of protective immunity had been induced by the vaccine.

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Documento generato il 02/12/20 alle ore 04:25:03