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Titolo:
REJECTION OF INTRAOCULAR TUMORS FROM TRANSGENIC MICE BY TUMOR-INFILTRATING LYMPHOCYTES
Autore:
MA D; ALIZADEH H; COMERFORD SA; GETHING MJ; SAMBROOK JF; ANAND R; NIEDERKORN JY;
Indirizzi:
UNIV TEXAS,SW MED CTR,DEPT OPHTHALMOL,5323 HARRY HINES BLVD DALLAS TX75235 UNIV TEXAS,SW MED CTR,DEPT OPHTHALMOL DALLAS TX 75235 HOWARD HUGHES MED INST DALLAS TX 75235 MCDERMOTT CTR HUMAN GROWTH & DEV DALLAS TX 75235
Titolo Testata:
Current eye research
fascicolo: 5, volume: 13, anno: 1994,
pagine: 361 - 369
SICI:
0271-3683(1994)13:5<361:ROITFT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
UVEAL MALIGNANT-MELANOMA; ANTIBODIES; MODEL; EYE;
Keywords:
TRANSGENIC MOUSE; INTRAOCULAR TUMOR; CYTOTOXIC T LYMPHOCYTE; REJECTION; CHOROID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
D. Ma et al., "REJECTION OF INTRAOCULAR TUMORS FROM TRANSGENIC MICE BY TUMOR-INFILTRATING LYMPHOCYTES", Current eye research, 13(5), 1994, pp. 361-369

Abstract

The present study examined the role of tumor infiltrating lymphocytes(TIL) in the rejection of intraocular tumors from SV40 transgenic mice. Tumor cells from an intraocular tumor arising in an SV40 transgenicFVB/N mouse were transplanted into the eyes of syngeneic FVB/N mice and the TIL isolated. TIL were assessed for direct cytolytic activity in vitro. TIL were also transferred passively to immunosuppressed FVB/Nmice to determine if they could mediate intraocular tumor rejection. The role of CD4(+) and CD8(+) T cells in intraocular tumor rejection was evaluated by depleting the respective cell populations in FVB/N hosts prior to intraocular tumor challenge. The results showed that intraocular tumors undergoing rejection in immunocompetent syngeneic hosts became infiltrated with T cells, with the CD8(+) subset predominating at the time of rejection. By contrast, athymic nude mice did not reject the intraocular tumors nor did the tumors become infiltrated with TIL. TIL displayed direct, tumor-specific cytolytic activity immediatelyafter isolation from the tumor-containing eyes. FVB/N hosts depleted of CD4(+) T cells were unable to reject their intraocular tumors. In vivo depletion of CD8(+) T cells delayed, but did not prevent tumor rejection. Adoptively transferred TIL mediated swift rejection of intraocular tumors in immunoincompetent recipients. Recipients of TIL, but not recipients of normal spleen cells, acquired significant tumor-specific CTL activity that was demonstrable in vitro. The results strongly suggest, but do not prove, that TIL mediate rejection of intraocular tumors from transgenic mice by direct cytolysis. Although CD4(+) T cellsare necessary for tumor rejection and are capable of direct cytolysis, the predominant effector cells are CD8(+) CTL.

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Documento generato il 01/12/20 alle ore 07:32:11