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Titolo:
INTERACTION WITH THE REV RESPONSE ELEMENT ALONG AN EXTENDED STEM-I DUPLEX STRUCTURE IS REQUIRED TO COMPLETE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REV-MEDIATED TRANSACTIVATION IN-VIVO
Autore:
KIMURA T; OHYAMA A;
Indirizzi:
KANSAI MED UNIV,DEPT MICROBIOL MORIGUCHI OSAKA 570 JAPAN MRC,MOLEC BIOL LAB CAMBRIDGE CB2 2QH ENGLAND
Titolo Testata:
Journal of Biochemistry
fascicolo: 5, volume: 115, anno: 1994,
pagine: 945 - 952
SICI:
0021-924X(1994)115:5<945:IWTRRE>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION REQUIRES; TOBACCO MOSAIC-VIRUS; VIRAL MESSENGER-RNA; HIV-1 REV; TARGET SEQUENCE; VIRION EXPRESSION; BINDING-SITE; PROTEIN; REGION; CELLS;
Keywords:
DUPLEX RNA; HIV-1; REV; RRE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
T. Kimura e A. Ohyama, "INTERACTION WITH THE REV RESPONSE ELEMENT ALONG AN EXTENDED STEM-I DUPLEX STRUCTURE IS REQUIRED TO COMPLETE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REV-MEDIATED TRANSACTIVATION IN-VIVO", Journal of Biochemistry, 115(5), 1994, pp. 945-952

Abstract

Expression of structural proteins of human immunodeficiency virus type 1 requires the direct interaction of the viral Rev traits-activator with its cis-acting RNA target sequence, the Rev response element (RRE). The originally defined 234-residue RRE, however, failed to show thefull HIV-1 regulator of virion expression (Rev) response when inserted into a test plasmid that lacked the flanking env sequences. Here, wedemonstrate that an alternative 351-residue complete RRE, which carries an extended Stem I structure, is required for the full Rev-responsiveness in vivo. Mutagenesis studies revealed that the association of Rev with RRE was initiated by the recognition of a functional bubble structure in stem-loop II, followed by interaction with the neighbouringduplex RNA, including the elongated Stem I structure. We propose that, as has been previously shown in vitro, the Rev-RRE interaction in vivo is an ordered assembly process and is based on the association of Rev molecules with the double-stranded region of RRE.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:16:26