Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IDENTIFICATION OF FUNCTIONAL DOMAINS OF ADENOVIRUS TUMOR-SPECIFIC TRANSPLANTATION ANTIGEN IN TYPE-5 AND TYPE-12 BY VIABLE VIRUSES CARRYING CHIMERIC E1A GENES
Autore:
SAWADA Y; RASKOVA J; FUJINAGA K; RASKA K;
Indirizzi:
ST PETERS MED CTR,INST MOLEC DIAGNOST & PATHOL NEW BRUNSWICK NJ 08903 ST PETERS MED CTR,INST MOLEC DIAGNOST & PATHOL NEW BRUNSWICK NJ 08903 UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT PATHOL PISCATAWAY NJ 08854 SAPPORO MED COLL,CANC RES INST,DEPT MOLEC BIOL SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
International journal of cancer
fascicolo: 4, volume: 57, anno: 1994,
pagine: 598 - 603
SICI:
0020-7136(1994)57:4<598:IOFDOA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOXIC LYMPHOCYTES-T; HIGHLY ONCOGENIC ADENOVIRUS-12; TRANSFORMED RAT-CELLS; NECROSIS-FACTOR-ALPHA; INFLUENZA NUCLEOPROTEIN; SYNTHETIC PEPTIDES; TUMORIGENICITY; EPITOPES; SENSITIVITY; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
Y. Sawada et al., "IDENTIFICATION OF FUNCTIONAL DOMAINS OF ADENOVIRUS TUMOR-SPECIFIC TRANSPLANTATION ANTIGEN IN TYPE-5 AND TYPE-12 BY VIABLE VIRUSES CARRYING CHIMERIC E1A GENES", International journal of cancer, 57(4), 1994, pp. 598-603

Abstract

The adenovirus (Ad) EIA gene induces in immunized animals a strong tumor transplantation (TSTA) immunity against Ad tumors. Such immunity with group-A and group-C viruses is highly group-specific and no cross-protection is detected between serotypes 5 and 12. This fact was used to map the domains of the Ad5 and Ad12 EIA gene products, respectively, which control the TSTA. We constructed a library of 8 recombinant viruses (H5sub1101 through H5sub1108) which carry chimeric Ad5/Ad12 EIA genes in the background of Ad5. The chimeric genes are functional and these viruses are viable. Some of these constructs induce strong and highly specific tumor syngraft immunity in immunized rats. The viruses carrying the 5' terminus of the first EIA exon derived from Ad12 (viruses H5sub1101, H5sub1102 and H5sub1103) induce strong protection against Ad12 tumors irrespective of the rest of their EIA sequence. The viruses which carry the second exon of the Ad5 EIA gene (viruses H5sub1101, H5sub1102 and H5sub1106) protect against group-C tumors, regardlessof the origin of the rest of their EIA gene. The 2 viruses that carrythe 5' EIA terminus of the first exon Ad12 and the second exon of Ad5(H5sub1101 and H5sub1102) are thus effective in inducing immunity against Ad12 tumors as well as against Ad2 tumors. The viruses which carry the 5' terminus of the first exon derived from Ad5 and the second exon of Ad12 (H5sub1107 and H5sub1108) fail to induce immunity against either tumor. Expression of only the truncated 5' terminus of the Ad12 EIA gene (viruses H5sub1104 and H5sub1105) is sufficient for inductionof Ad12 TSTA. Our results provide direct and unequivocal in vivo evidence that TSTA activities of adenovirus groups A and C are controlled by different domains of their respective EIA genes. The Ad12 TSTA is afunction of the 5' terminus of the first EIA exon, while the Ad5 TSTAis coded for by the 3' exon of its EIA gene. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 19:45:03