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Titolo:
HYDROGEN-BOND DETECTION IN PEPTIDES BY H-1-NUCLEAR MAGNETIC-RESONANCETHROUGH A HYDROGEN-CHLORINE EXCHANGE-REACTION
Autore:
KONDO M; OKAMOTO K; NISHI I; YAMAMOTO M; JELOKHANINIARAKI M; KODAMA H;
Indirizzi:
SAGA UNIV,FAC SCI & ENGN,DEPT CHEM SAGA 840 JAPAN KYUSHU INST TECHNOL,DEPT BIOCHEM ENGN & SCI IIZUKA 820 JAPAN
Titolo Testata:
Bulletin of the Chemical Society of Japan
fascicolo: 4, volume: 67, anno: 1994,
pagine: 1064 - 1068
SICI:
0009-2673(1994)67:4<1064:HDIPBH>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-CHLOROANILINES; RIBONUCLEASE-A; AMIDE; REARRANGEMENT; CONFORMATION; PROTONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
M. Kondo et al., "HYDROGEN-BOND DETECTION IN PEPTIDES BY H-1-NUCLEAR MAGNETIC-RESONANCETHROUGH A HYDROGEN-CHLORINE EXCHANGE-REACTION", Bulletin of the Chemical Society of Japan, 67(4), 1994, pp. 1064-1068

Abstract

NMR spectroscopy is a versatile method for the conformational analysis of peptides and proteins. The hydrogen-chlorine exchange of amide NHprotons is detected by H-1 NMR and used as a method to distinguish between intramolecularly hydrogen-bonded and solvent-exposed NH moieties. The method has been applied to hydrogen bond detection in naturally occurring antibiotic peptides, such as gramicidin S, and CH3CONH-X (X = alkyl- or aryl-) derivatives. The deuterium exchange method was compared with this method in parallel experiments. In the case of chlorineexchange, in contrast to deuterium exchange, the hydrogen-bonded amide protons are replaced much faster than their solvent-exposed counterparts and the duration of the experiments is considerably less. It is highly possible that the hydrogen-chlorine exchange reaction under the present experimental conditions, in the dark and at room temperature, proceeds through an electrophilic polar mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:47:50