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Titolo:
NATURAL HUMAN INTERFERON-ALPHA AUGMENTS APOPTOSIS IN ACTIVATED T-CELLLINE
Autore:
DAO T; ARIYASU T; HOLAN V; MINOWADA J;
Indirizzi:
HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR,675-1 FUJISAKI OKAYAMA702 JAPAN ACAD SCI CZECH REPUBL,INST MOLEC GENET CR-16637 PRAGUE 6 CZECH REPUBLIC
Titolo Testata:
Cellular immunology
fascicolo: 2, volume: 155, anno: 1994,
pagine: 304 - 311
SICI:
0008-8749(1994)155:2<304:NHIAAI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CD3/T-CELL RECEPTOR COMPLEX; DNA FRAGMENTATION; INTERLEUKIN-2 PRODUCTION; HUMAN-LYMPHOCYTES; ENTEROTOXIN-B; DEATH; ANTIGEN; ANTIBODIES; GAMMA; THYMOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
T. Dao et al., "NATURAL HUMAN INTERFERON-ALPHA AUGMENTS APOPTOSIS IN ACTIVATED T-CELLLINE", Cellular immunology, 155(2), 1994, pp. 304-311

Abstract

We have previously shown that interferon-alpha (IFN-alpha) augments interleukin-2 (IL-2) production in mitogen-activated but not unstimulated T cells (1). Here, we studied the effect of IFN-alpha on activation-driven cell death (apoptosis) using a human leukemia T cell line, MOLT-16, as a T cell activation model. IFN-alpha alone had no effect on either the IL-2 production or apoptosis of MOLT-16 cells, but significantly increased both the IL-2 production and apoptosis in the MOLT-16 cells after phytohemagglutinin (PHA) stimulation as determined by CTLL-2 assay and by flow cytometric analysis using propidium iodide (PI) staining. Since IL-2 has been shown to induce apoptosis in some systems,we next evaluated whether the apoptosis in MOLT-16 cells was due to endogenous IL-2 production upon PHA stimulation. However, the addition of exogenous IL-2 to unstimulated cultures of MOLT-16 did not induce DNA fragmentation, a characteristic feature of apoptosis, as determinedby DNA electrophoresis and by flow cytometric analysis with PI-stained cells. Furthermore, anti-IL-2 antibody did not prevent PHA-induced DNA fragmentation in MOLT-16 cells. Thus, we conclude that both PHA-induced IL-2 production and apoptosis are outcomes of T cell activation and that IFN-alpha may exert immunoregulatory effects on T cell activation by augmenting both processes. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 16:08:05