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Titolo:
HEPATOCYTE GROWTH-FACTOR PROMOTES MOTOR-NEURON SURVIVAL AND SYNERGIZES WITH CILIARY NEUROTROPHIC FACTOR
Autore:
WONG VV; GLASS DJ; ARRIAGA R; YANCOPOULOS GD; LINDSAY RM; CONN G;
Indirizzi:
REGENERON PHARMACEUT INC,777 OLD SAW MILL RIVER RD TARRYTOWN NY 10591
Titolo Testata:
The Journal of biological chemistry
fascicolo: 8, volume: 272, anno: 1997,
pagine: 5187 - 5191
SICI:
0021-9258(1997)272:8<5187:HGPMSA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT SKELETAL-MUSCLE; SCATTER FACTOR; C-MET; POSTMITOTIC NEURONS; TYROSINE KINASE; FACTOR RECEPTOR; MAH CELLS; LIMB BUD; VINCRISTINE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
V.V. Wong et al., "HEPATOCYTE GROWTH-FACTOR PROMOTES MOTOR-NEURON SURVIVAL AND SYNERGIZES WITH CILIARY NEUROTROPHIC FACTOR", The Journal of biological chemistry, 272(8), 1997, pp. 5187-5191

Abstract

Hepatocyte growth factor (HGF) has been shown to function as a potentmitogen for a variety of cells, transducing its signal through the c-met tyrosine kinase receptor. Ciliary neurotrophic factor (CNTF) is a cytokine that has been shown to promote survival of motor neurons. We show here that c-met mRNA is present in the embryonic rat spinal cord. Peak expression of c-met (at E14) coincides with the period of naturally occurring cell death in motor neurons, suggesting a possible role of HGF in the regulation of this process. Utilizing a neuron-enriched culture system, we established that HGF, like CNTF, stimulates cholineacetyltransferase (CAT) activity in motor neurons. When co administered to motor neuron cultures, saturating concentrations of HGF and CNTFproduced a synergistic increase in CAT levels. We show that this synergy reflects enhanced motor neuron survival. Exposure of motor neuron cultures to the cytostatic agent vincristine markedly decreased CAT levels; co treatment with HGF and CNTF (but not either factor alone) restored CAT activity to control levels. Our findings indicate that HGF is a survival factor for motor neurons, that it acts synergistically with CNTF, and that HGF and CNTF can together be neuroprotective in the face of vincristine toxicity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 20:08:22