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Titolo:
DIFFERENTIAL EXPRESSION OF CHITINASE AND BETA-1,3-GLUCANASE GENES IN VARIOUS TISSUES OF POTATO PLANTS
Autore:
GARCIAGARCIA F; SCHMELZER E; HAHLBROCK K; ROXBY R;
Indirizzi:
UNIV MAINE,DEPT BIOCHEM MICROBIOL & MOLEC BIOL ORONO ME 04469 UNIV MAINE,DEPT BIOCHEM MICROBIOL & MOLEC BIOL ORONO ME 04469 MAX PLANCK INST ZUCHTUNGSFORSCH,BIOCHEM ABT D-50829 COLOGNE 30 GERMANY
Titolo Testata:
Zeitschrift fur Naturforschung. C, A journal of biosciences
fascicolo: 3-4, volume: 49, anno: 1994,
pagine: 195 - 203
SICI:
0939-5075(1994)49:3-4<195:DEOCAB>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGENESIS-RELATED PROTEINS; PHENYLALANINE AMMONIA-LYASE; ETHYLENE-INDUCED CHITINASE; PHYTOPHTHORA-INFESTANS; SPATIAL PATTERNS; MESSENGER-RNA; BEAN-LEAVES; TOBACCO; ENZYME; LOCALIZATION;
Keywords:
SOLANUM TUBEROSUM; POLYSACCHARIDE METABOLISM; IN SITU MESSENGER-RNA HYBRIDIZATION; IMMUNOHISTOCHEMISTRY; PHENYLALANINE AMMONIA-LYASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
F. Garciagarcia et al., "DIFFERENTIAL EXPRESSION OF CHITINASE AND BETA-1,3-GLUCANASE GENES IN VARIOUS TISSUES OF POTATO PLANTS", Zeitschrift fur Naturforschung. C, A journal of biosciences, 49(3-4), 1994, pp. 195-203

Abstract

We have analyzed the tissue-specific expression of chitinase, beta-1,3-glucanase and, for comparison, phenylalanine ammonia-lyase genes in healthy, unstressed potato (Solanum tuberosum) plants by using RNA-RNAhybridization and immunostaining techniques to localize the respective mRNAs and proteins in situ. Preparations of leaf, newer, shoot tip, node and axillary bud revealed that chitinase exhibited a distinctive and characteristic expression pattern in being confined to the epidermal cell layer, while beta-1,3-glucanase and phenylalanine ammonia-lyase were present to varying extents in most tissues of these organs. These distinctive patterns of expression suggest fundamentally different roles for the two glucanohydrolases in the metabolism of unstressed plants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 21:46:13