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Titolo:
CYTOKINE-INDUCED AND ENDOTOXIN-INDUCED NITRIC-OXIDE SYNTHASE IN RAT ASTROGLIAL CULTURES - DIFFERENTIAL MODULATION BY ANGIOTENSIN-II
Autore:
KOPNISKY KL; SUMNERS C; CHANDLER LJ;
Indirizzi:
UNIV FLORIDA,COLL MED,DEPT PHYSIOL GAINESVILLE FL 32611 UNIV FLORIDA,COLL MED,DEPT PHYSIOL GAINESVILLE FL 32611 LOUISIANA STATE UNIV,MED CTR,DEPT PHARMACOL SHREVEPORT LA 00000
Titolo Testata:
Journal of neurochemistry
fascicolo: 3, volume: 68, anno: 1997,
pagine: 935 - 944
SICI:
0022-3042(1997)68:3<935:CAENSI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; NECROSIS-FACTOR-ALPHA; GENE-EXPRESSION; INTERFERON-GAMMA; GLIAL CULTURES; MESSENGER-RNA; INDUCTION; ASTROCYTES; BRAIN; CELLS;
Keywords:
ANGIOTENSIN II; ASTROGLIA; LIPOPOLYSACCHARIDE; INTERFERON-GAMMA; INTERLEUKIN-1-BETA; NITRIC OXIDE SYNTHASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
K.L. Kopnisky et al., "CYTOKINE-INDUCED AND ENDOTOXIN-INDUCED NITRIC-OXIDE SYNTHASE IN RAT ASTROGLIAL CULTURES - DIFFERENTIAL MODULATION BY ANGIOTENSIN-II", Journal of neurochemistry, 68(3), 1997, pp. 935-944

Abstract

Recent studies have shown that the stimulatory effects of bacterial endotoxin [lipopolysaccharide (LPS)] on inducible nitric oxide (NO) synthase (iNOS) in astroglia are significantly reduced by the peptide angiotensin II (Ang II). In the present study we have compared the modulatory actions of Ang II on cytokine- and LPS-stimulated iNOS in astroglia cultured from adult rat brain. Incubation of astroglia with LPS (100 ng/ml; 24 h) and/or combinations of interleukin-1 beta (IL-1 beta; 10 ng/ml, 24 h), interferon-gamma (IFN-gamma; 100 U/ml, 24 h), or tumornecrosis factor-alpha (TNF-alpha; 100 ng/ml, 24 h) resulted in significant increases of iNOS mRNA, iNOS protein, and NO production, with the latter indicated by increased nitrite accumulation. The effects of LPS, IL-1 beta, and TNF-alpha were significantly decreased by coincubation with Ang II (100 ng/ml, 24 h). In contrast, Ang II did not alter the stimulation of iNOS mRNA levels and NO production elicited by IFN-gamma. Therefore, Ang II differentially modulates the stimulatory actions of LPS and cytokines on iNOS, and subsequently NO production, in astroglia. These data suggest that Ang II may have an important modulatory role in intracerebral immune responses that involve production of NO by astroglia.

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Documento generato il 02/12/20 alle ore 16:53:42