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Titolo:
CRYOPRESERVATION OF IN-VITRO GROWN APICAL MERISTEMS OF WASABI (WASABIA-JAPONICA) BY VITRIFICATION AND SUBSEQUENT HIGH PLANT-REGENERATION
Autore:
MATSUMOTO T; SAKAI A; YAMADA K;
Indirizzi:
SHIMANE AGR EXPTL STN IZUMO SHIMANE 693 JAPAN
Titolo Testata:
Plant cell reports
fascicolo: 8, volume: 13, anno: 1994,
pagine: 442 - 446
SICI:
0721-7714(1994)13:8<442:COIGAM>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
VAR BRASILIENSIS TANAKA; SHOOT TIPS; NUCELLAR CELLS;
Keywords:
CRYOPRESERVATION; APICAL MERISTEMS VITRIFICATION; WASABI (WASABIA JAPONICA);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
T. Matsumoto et al., "CRYOPRESERVATION OF IN-VITRO GROWN APICAL MERISTEMS OF WASABI (WASABIA-JAPONICA) BY VITRIFICATION AND SUBSEQUENT HIGH PLANT-REGENERATION", Plant cell reports, 13(8), 1994, pp. 442-446

Abstract

In vitro-grown apical meristems of wasabi (Wasabia japonica Matsumura) were successfully cryopreserved by vitrification. Excised apical meristems precultured on solidified MS medium containing 0.3M sucrose at 20 degrees C for 1 day were loaded with a mixture of 2M glycerol and 0.4M sucrose for 20 min at 25 degrees C. Cryoprotected meristems were then sufficiently dehydrated with a highly concentrated vitrification solution (designated PVS2) for 10 min at 25 degrees C prior to a plungeinto liquid nitrogen. After rapid warming, the meristems were expelled into 2 ml of 1.2M sucrose for 20 min and then plated on solidified culture medium. Successfully vitrified and warmed meristems remained green after plating, resumed growth within 3 days, and directly developed shoots within two weeks. The average rate of normal shoot formation amounted to about 80 to 90% in the cryopreserved meristems. This method was successfully applied to three other cultivars of wasabi. This vitrification procedure promises to become a routine method for cryopreserving meristems of wasabi.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:05:51