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Titolo:
SLOWLY CYCLING (LABEL-RETAINING) EPIDERMAL-CELLS BEHAVE LIKE CLONOGENIC STEM-CELLS IN-VITRO
Autore:
MORRIS RJ; POTTEN CS;
Indirizzi:
LANKENAU MED RES CTR,100 LANCASTER AVE W CITY LINE WYNNEWOOD PA 19096 UNIV TEXAS,MD ANDERSON CANC CTR SMITHVILLE TX 78957 CHRISTIE HOSP,NHS TRUST,PATERSON INST CANC RES MANCHESTER ENGLAND
Titolo Testata:
Cell proliferation
fascicolo: 5, volume: 27, anno: 1994,
pagine: 279 - 289
SICI:
0960-7722(1994)27:5<279:SC(EBL>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE SKIN; 2-STAGE CARCINOGENESIS; PROLIFERATIVE UNIT; TUMOR INITIATION; MURINE EPIDERMIS; STRATUM CORNEUM; PROMOTION; KERATINOCYTES; IDENTIFICATION; PERSISTENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Morris e C.S. Potten, "SLOWLY CYCLING (LABEL-RETAINING) EPIDERMAL-CELLS BEHAVE LIKE CLONOGENIC STEM-CELLS IN-VITRO", Cell proliferation, 27(5), 1994, pp. 279-289

Abstract

Slowly cycling label-retaining epidermal cells were identified by light microscopic autoradiography in the dorsal epidermis and hair follicles of adult mice 8-10 weeks after twice daily injection of [H-3]dT ondays three through five after birth. Pulse-labelled epidermal cells were identified in the epidermis and hair follicles of 7-8 week old mice 1 h after a single injection of [H-3]dT at 8.00 a.m. For mice of both groups, epidermal cells including those from the hair follicles wereharvested by trypsinization and were cultured from low density on feeder layers of irradiated Swiss mouse 3T3. On days 2, 4, 5, 7, 10 and 12, the cultures were fixed and processed for light microscopic autoradiography, and the distribution of labelled nuclei was quantified. On day 2 of culture, both label-retaining cells (LRC) and pulse labelled cells (PLC) were found primarily as single cells. After five days, LRC were found as pairs and clusters having silver grain counts consistentwith their division. In contrast, PLC remained primarily as single cells. These results suggest that LRC may divide to form colonies (are clonogenic) whereas PLC are rarely clonogenic. The significance of thisexperiment is that it suggests that the LRC may not only be persistent in the epidermis, but that they may also be cells with relatively greater proliferative potential than the PLC and are thus likely to be stem cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:29:26