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Titolo:
IDENTIFICATION OF A LIGHT-RESPONSIVE REGION OF THE NUCLEAR GENE ENCODING THE B-SUBUNIT OF CHLOROPLAST GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM ARABIDOPSIS-THALIANA
Autore:
KWON HB; PARK SC; PENG HP; GOODMAN HM; DEWDNEY J; SHIH MC;
Indirizzi:
UNIV IOWA,DEPT SCI BIOL IOWA CITY IA 52242 UNIV IOWA,DEPT SCI BIOL IOWA CITY IA 52242 HARVARD UNIV,SCH MED,DEPT GENET BOSTON MA 02114 MASSACHUSETTS GEN HOSP,DEPT MOLEC BIOL BOSTON MA 02114
Titolo Testata:
Plant physiology
fascicolo: 1, volume: 105, anno: 1994,
pagine: 357 - 367
SICI:
0032-0889(1994)105:1<357:IOALRO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING PROTEIN; RIBULOSE-BISPHOSPHATE CARBOXYLASE; POLYMERASE CHAIN-REACTION; DIFFERENTIAL EXPRESSION; MESSENGER-RNA; HIGHER-PLANTS; RBCS-3A GENE; PROMOTER; SEQUENCES; ELEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
H.B. Kwon et al., "IDENTIFICATION OF A LIGHT-RESPONSIVE REGION OF THE NUCLEAR GENE ENCODING THE B-SUBUNIT OF CHLOROPLAST GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM ARABIDOPSIS-THALIANA", Plant physiology, 105(1), 1994, pp. 357-367

Abstract

We report here the identification of a cis-acting region involved in light regulation of the nuclear gene (GapB) encoding the B subunit of chloroplast glyceraldehyde 3-phosphate dehydrogenase from Arabidopsis thaliana. Our results show that a 664-bp GapB promoter fragment is sufficient to confer light induction and organ-specific expression of theEscherichia coli B-glucuronidase reporter gene (Gus) in transgenic tobacco (Nicotiana tabacum) plants. Deletion analysis indicates that the-261 to -173 upstream region of the GapB gene is essential for light induction. This region contains four direct repeats with the consensussequence 5'-ATGAA(A/G)A-3' (Gap boxes). Deletion of all four repeats abolishes light induction completely. In addition, we have linked a 109-bp (-263 to -152) GapB upstream fragment containing the four direct repeats in two orientations to the -92 to +6 upstream sequence of the cauliflower mosaic virus 35S basal promoter. The resulting chimeric promoters are able to center light induction and to enhance leaf-specific expression of the Gus reporter gene in transgenic tobacco plants. Based on these results we conclude that Cap boxes are essential for light regulation and organ-specific expression of the GapB gene in A. thaliana. Using gel mobility shift assays we have also identified a nuclear factor from tobacco that interacts with GapA and GapB DNA fragments containing these Cap boxes. Competition assays indicate that Cap boxesare the binding sites for this factor. Although this binding activityis present in nuclear extracts from leaves and roots of light-grown or dark-treated tobacco plants, the activity is less abundant in nuclear extracts prepared from leaves of dark-treated plants or from roots of greenhouse-grown plants. In addition, our data show that this binding factor is distinct from the GT-1 factor, which binds to Box II and Box III within the light-responsive element of the RbcS-3A gene of pea.

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Documento generato il 30/11/20 alle ore 00:30:41