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Titolo:
STRESS-RESPONSE OF PORPHYROMONAS-GINGIVALIS
Autore:
LU B; MCBRIDE BC;
Indirizzi:
UNIV BRITISH COLUMBIA,DEPT MICROBIOL,300-6174 UNIV BLVD VANCOUVER V6T1Z3 BC CANADA UNIV BRITISH COLUMBIA,DEPT MICROBIOL & IMMUNOL VANCOUVER BC CANADA UNIV BRITISH COLUMBIA,DEPT ORAL BIOL VANCOUVER BC CANADA
Titolo Testata:
Oral microbiology and immunology
fascicolo: 3, volume: 9, anno: 1994,
pagine: 166 - 173
SICI:
0902-0055(1994)9:3<166:SOP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; OUTER-MEMBRANE PROTEINS; BACTEROIDES-GINGIVALIS; ESCHERICHIA-COLI; CLOSTRIDIUM-ACETOBUTYLICUM; PERIODONTAL-DISEASE; ENZYME-ACTIVITY; W50; HEMIN; DNAK;
Keywords:
PORPHYROMONAS GINGIVALIS; ANAEROBE; STRESS; HEAT SHOCK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
B. Lu e B.C. Mcbride, "STRESS-RESPONSE OF PORPHYROMONAS-GINGIVALIS", Oral microbiology and immunology, 9(3), 1994, pp. 166-173

Abstract

The heat shock response of Porphyromonas gingivalis was examined by 1- and 2-dimensional polyacrylamide gel electrophoresis after metaboliclabeling with [C-14]-amino acids. When P. gingivalis cells were shifted from 37 degrees C to 42 degrees C, elevated synthesis of 4 proteinswith the apparent molecular weight of 92, 80, 74, and 62 kDa was observed, and synthesis of a 50-kDa protein decreased during heat shock. The 74- and 62-kDa proteins were identified as homologs of Escherichia coli DnaK and GroEL respectively by Western immunoblotting. On 2-dimensional Western blots, 2 forms of DnaK and 4 forms of GroEL were identified due to their slightly different isolelectric points. dnaK and groEL gene homologs were identified in several P. gingivalis strains and some other black-pigmented oral species. DnaK and GroEL homolog proteins were induced when P. gingivalis cells were challenged by ethanol. These proteins were not induced by oxidative stress or change in pH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:24:50