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Titolo:
ENZYMES IN DIAGNOSTICS - ACHIEVEMENTS AND POSSIBILITIES OF RECOMBINANT-DNA TECHNOLOGY
Autore:
KOPETZKI E; LEHNERT K; BUCKEL P;
Indirizzi:
BOEHRINGER MANNHEIM GMBH,DEPT GENET,NONNENWALD 2 D-82377 PENZBERG GERMANY
Titolo Testata:
Clinical chemistry
fascicolo: 5, volume: 40, anno: 1994,
pagine: 688 - 704
SICI:
0009-9147(1994)40:5<688:EID-AA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST ALPHA-GLUCOSIDASE; ESCHERICHIA-COLI; ASPERGILLUS-NIGER; SACCHAROMYCES-CEREVISIAE; GENE FUSION; GALACTOSE DEHYDROGENASE; ALKALINE-PHOSPHATASE; PROTEIN GLYCOSYLATION; BIFUNCTIONAL ENZYME; SEQUENCE-ANALYSIS;
Keywords:
RECOMBINANT ENZYMES; ENZYME PURIFICATION; ENZYME IMMUNOASSAY; ENZYME AMPLIFICATION; ENZYME IMMOBILIZATION;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
96
Recensione:
Indirizzi per estratti:
Citazione:
E. Kopetzki et al., "ENZYMES IN DIAGNOSTICS - ACHIEVEMENTS AND POSSIBILITIES OF RECOMBINANT-DNA TECHNOLOGY", Clinical chemistry, 40(5), 1994, pp. 688-704

Abstract

We discuss, from an industrial point of view, the scope and possibilities of recombinant DNA technology for ''diagnostic enzyme'' production and application. We describe the construction of enzyme-overproducing strains and show how to simplify downstream processing, increase product quality and process profitability, improve diagnostic enzyme properties, and adjust enzymes to harsh assay conditions. We also considersome safety and environmental aspects of enzyme production. Other aspects of diagnostic enzymes that we cover are the facilitation of enzyme purification by attachment of short amino acid tails, the introduction of tails or tags for site-specific conjugation or oriented immobilization, the construction of bi- or multifunctional enzymes, and the production of enzyme-based diagnostic tests as demonstrated by the homogeneous immunoassay system of CEDIA(R) tests. We use as examples of diagnostic enzymes glucose-6-phosphate dehydrogenase (EC 1.1.1.49), glucose oxidase (EC 1.1.3.4), alkaline phosphatase (EC 3.1.3.1), alpha-glucosidase (EC 3.2.1.20), pyruvate oxidase (EC 1.2.3.3), creatinase (EC 3.5.3.3), and beta galactosidase (EC 3.2.1.23).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 05:05:12