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Titolo:
CELLULAR PROTEIN MODULATES EFFECTS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REV
Autore:
LUO Y; YU HF; PETERLIN BM;
Indirizzi:
UNIV CALIF SAN FRANCISCO,DEPT MED,BOX 0724,3RD & PARNASSUS AVE SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT MED SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT MED SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL SAN FRANCISCO CA 94143
Titolo Testata:
Journal of virology
fascicolo: 6, volume: 68, anno: 1994,
pagine: 3850 - 3856
SICI:
0022-538X(1994)68:6<3850:CPMEOH>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION REQUIRES; RNA RESPONSE ELEMENTS; SPLICE SITE SELECTION; VIRAL MESSENGER-RNA; HTLV-I REX; HIV-1 REV; TRANS-ACTIVATOR; TARGET SEQUENCE; BINDING; REGULATORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
Y. Luo et al., "CELLULAR PROTEIN MODULATES EFFECTS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REV", Journal of virology, 68(6), 1994, pp. 3850-3856

Abstract

Replication of human immunodeficiency virus type 1 requires expression of the viral trans activator Rev. Rev binds to a highly structured RNA, the Rev response element, which is present in singly spliced and unspliced genomic viral RNAs. Although Rev helps to transport these transcripts from the nucleus to the cytoplasm, the mechanism(s) involved is not fully understood. Using the yeast two-hybrid system, we isolated a murine protein (YL2) that interacts with the basic domain of Rev, which is essential for the function of Rev in vivo and for the inhibitory splicing activity of Rev in vitro. YL2 has 92% identity to a human32-kDa protein (p32), which copurifies with alternative splicing factor SF2/ASF. Furthermore, we found that whereas expression of YL2 greatly potentiated the activity of Rev, antisense YL2 transcripts blocked the effects of Rev in mammalian cells. YL2 also increased the activities of Res on the Res response element and of hybrid Rev proteins fusedto Tat and the coat protein of bacteriophage MS2 on their respective RNAs. Thus, YL2 or p32 is a cellular protein that modulates the function of human immunodeficiency virus type 1 Rev.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 07:58:27