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Titolo:
EFFECT OF ENDOTHELIN-1 AS GROWTH-FACTOR ON A HUMAN GLIOMA CELL-LINE -ITS CHARACTERISTIC PROMOTION OF DNA-SYNTHESIS
Autore:
ASANO T; AOYAGI M; HIRAKAWA K; IKAWA Y;
Indirizzi:
TOKYO MED & DENT UNIV,SCH MED,DEPT NEUROSURG,BUNKYO KU,1-5-45 YUSHIMATOKYO 113 JAPAN TOKYO MED & DENT UNIV,SCH MED,DEPT BIOCHEM TOKYO 113 JAPAN
Titolo Testata:
Journal of neuro-oncology
fascicolo: 1, volume: 18, anno: 1994,
pagine: 1 - 7
SICI:
0167-594X(1994)18:1<1:EOEAGO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBRAL VASOSPASM; BRAIN; MITOGENESIS; RECEPTORS; RAT;
Keywords:
ENDOTHELIN; HUMAN GLIOMA CELLS; DNA SYNTHESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
T. Asano et al., "EFFECT OF ENDOTHELIN-1 AS GROWTH-FACTOR ON A HUMAN GLIOMA CELL-LINE -ITS CHARACTERISTIC PROMOTION OF DNA-SYNTHESIS", Journal of neuro-oncology, 18(1), 1994, pp. 1-7

Abstract

In addition to its powerful vasoconstrictive activity, endothelin-l (ET-1) has been recognized to stimulate DNA synthesis in some cell lines. In this study, we confirmed the existence of ET-1 receptor in YKG-1human glioma cells, and investigated its effect on DNA synthesis in YKG-1 for 6 consecutive days, comparing it with that of epidermal growth factor (EGF), platelet-derived growth factor (PDGF), and insulin-like growth factor-I (IGF-I). Scatchard analysis of the binding data revealed the presence of a single class of high-affinity binding molecule. The apparent dissociation constant (Kd) was 5.2 x 10(-9) M and the maximal binding capacity (B max) was 4.7 x 10(4) sites/cell. The percentage of non-cycling cells was initially more than 85 %, and decreased to 55.40 %, 24.22 %, 11.50 %, and 7.51 % on days 1, 2, 4, and 6, respectively, after ET-1 stimulation. Although ET-I reduces the fraction of non-cycling cells more slowly than other growth factors such as EGF, PDGF and IGF-I, it reaches the same level as the others by day 6. Theseresults indicate that YKG-1 human glioma cells have ET-1 receptors and that ET-1 initiates a peculiar slow induction of DNA synthesis in these cells, suggesting that secondary factors might exist to acceleratethe DNA synthesis in response to ET-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 20:14:43