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Titolo:
CLONING, SEQUENCING AND DEDUCED FUNCTIONS OF A CLUSTER OF STREPTOMYCES GENES PROBABLY ENCODING BIOSYNTHESIS OF THE POLYKETIDE ANTIBIOTIC FRENOLICIN
Autore:
BIBB MJ; SHERMAN DH; OMURA S; HOPWOOD DA;
Indirizzi:
JOHN INNES INST,JOHN INNES CTR NORWICH NR4 7UH NORFOLK ENGLAND JOHN INNES INST,JOHN INNES CTR NORWICH NR4 7UH NORFOLK ENGLAND KITASATO INST,MINATO KU TOKYO 108 JAPAN
Titolo Testata:
Gene
fascicolo: 1, volume: 142, anno: 1994,
pagine: 31 - 39
SICI:
0378-1119(1994)142:1<31:CSADFO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE; COELICOLOR A3(2); SYNTHASE; GLAUCESCENS; VECTORS; DNA; VIOLACEORUBER; ACTINORHODIN; CONSTRUCTION; REPLACEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
M.J. Bibb et al., "CLONING, SEQUENCING AND DEDUCED FUNCTIONS OF A CLUSTER OF STREPTOMYCES GENES PROBABLY ENCODING BIOSYNTHESIS OF THE POLYKETIDE ANTIBIOTIC FRENOLICIN", Gene, 142(1), 1994, pp. 31-39

Abstract

A 10.2-kb fragment of DNA from Streptomyces roseofulvus, which contains polyketide synthase(PKS)-encoding genes (fr en) presumed to determine production of the antibiotics frenolicin and the nanaomycins, was cloned. A 5530-bp continuous segment of this DNA was sequenced. Analysis of the sequence revealed five complete open reading frames (ORFs) transcribed in one direction (ORFs 1, 2, 3, 5, 4.) and one (ORFX), located between ORF3 and ORF5, transcribed in the opposite direction. The deduced amino-acid sequences of ORFs 1, 2, 3, 4 and 5 closely resemble the sequences of known Components of the type-II PKS from other Streptomyces species: putative heterodimeric (ORF1+2) ketosynthase, acyl carrier protein, cyclase and ketoreductase, respectively. A resemblance between the N-terminal and C-terminal halves of the ORF4 product - alsodiscovered in the corresponding genes from other isochromanequinone antibiotic producers - suggests a possible origin of the cyclase-encoding gene by duplication. ORFX appears to represent a novel class of genes of unknown function present not only in the fren cluster, but also in other clusters of aromatic antibiotic biosynthetic genes in Streptomyces species. The fren-ORF1-5 genes, encoding a PKS that constructs anascent polyketide of either 16 or 18 carbons, compared with fixed lengths of 16 and 20 for other available examples, are proving to be valuable for understanding the mechanisms controlling polyketide chain length and patterns of reduction and cyclisation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 12:49:19