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Titolo:
EFFECTS OF PENTAMIDINE ISETHIONATE ON SACCHAROMYCES-CEREVISIAE
Autore:
LUDEWIG G; WILLIAMS JM; LI Y; STABEN C;
Indirizzi:
UNIV KENTUCKY,TH MORGAN SCH BIOL SCI,101 MORGAN BLDG LEXINGTON KY 40506 UNIV KENTUCKY,TH MORGAN SCH BIOL SCI LEXINGTON KY 40506
Titolo Testata:
Antimicrobial agents and chemotherapy
fascicolo: 5, volume: 38, anno: 1994,
pagine: 1123 - 1128
SICI:
0066-4804(1994)38:5<1123:EOPIOS>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PNEUMOCYSTIS-CARINII PNEUMONIA; INHIBITION; INVITRO; GENE; SENSITIVITY; EXPRESSION; SEQUENCE; COMPLEX; BRUCEI; ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
G. Ludewig et al., "EFFECTS OF PENTAMIDINE ISETHIONATE ON SACCHAROMYCES-CEREVISIAE", Antimicrobial agents and chemotherapy, 38(5), 1994, pp. 1123-1128

Abstract

We used Saccharomyces cerevisiae as a model system in which to examine the mechanism of action of the anti-Pneumocystis drug pentamidine. Pentamidine at low concentrations inhibited S. cerevisiae growth on nonfermentable carbon sources (50% inhibitory concentration [IC50] of 1.25 mu g/ml in glycerol). Pentamidine inhibited growth on fermentable energy sources only at much higher concentrations (IC50 of 250 mu g/ml in glucose). Inhibition at low pentamidine concentrations in glycerol was due to cytostatic activity rather than cytotoxic or mutagenic activity. Pentamidine also rapidly inhibited respiration by intact yeast cells, although inhibitory concentrations were much higher than those inhibitory to growth (IC50 of 100 mu g/ml for respiration). Pentamidine also induced petite mutations, although only at concentrations much higher than those required for growth inhibition. These results suggest that a function essential for respiratory growth is inhibited by pentamidine and that pentamidine affects mitochondrial processes, We propose the hypothesis that the primary cellular target of pentamidine in S.cerevisiae is the mitochondrion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 13:29:35