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Titolo:
H-1-NMR STUDIES OF THE HIGH-AFFINITY REV BINDING-SITE OF THE REV RESPONSIVE ELEMENT OF HIV-1 MESSENGER-RNA - BASE FAIRING IN THE CORE BINDING-ELEMENT
Autore:
PETERSON RD; BARTEL DP; SZOSTAK JW; HORVATH SJ; FEIGON J;
Indirizzi:
UNIV CALIF LOS ANGELES,DEPT CHEM & BIOCHEM LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,DEPT CHEM & BIOCHEM LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,INST MOLEC BIOL LOS ANGELES CA 90024 MASSACHUSETTS GEN HOSP,DEPT MOLEC BIOL BOSTON MA 02114 CALTECH,DIV BIOL PASADENA CA 91125
Titolo Testata:
Biochemistry
fascicolo: 18, volume: 33, anno: 1994,
pagine: 5357 - 5366
SICI:
0006-2960(1994)33:18<5357:HSOTHR>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; GENE-EXPRESSION REQUIRES; VIRAL MESSENGER-RNA; TARGET SEQUENCE; VIRION EXPRESSION; TRANS-ACTIVATOR; PROTEIN; SPECTROSCOPY; CONFORMATION; ADJACENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
R.D. Peterson et al., "H-1-NMR STUDIES OF THE HIGH-AFFINITY REV BINDING-SITE OF THE REV RESPONSIVE ELEMENT OF HIV-1 MESSENGER-RNA - BASE FAIRING IN THE CORE BINDING-ELEMENT", Biochemistry, 33(18), 1994, pp. 5357-5366

Abstract

H-1 NMR studies of a 30-nucleotide RNA oligonucleotide (RBE3), which contains a high-affinity binding site for Rev of the HIV-1 Rev responsive element (RRE), two derivatives of RBE3 (RBE3AA and RBE3-A), and the complex of RBE3 with peptides derived from the RNA binding domain ofHIV-1 Rev, are presented. The high-affinity binding site of the RRE consists of an asymmetric internal loop and surrounding Watson-Crick base pairs. In the wild-type RRE, one of the stems is closed by a loop; this is replaced in REB3 by the stable UUCG tetraloop. NOE data suggest that the internal loop of the free RNA contains structural features that have been predicted on the basis of in vitro selection experiments [Bartel, D. P., et al. (1991) Cell 67, 529-536]. The structural features include a G(syn).G(anti) base pair, a G(anti).A(anti) base pair, and a looped out U. When the Rev peptide is bound to the RNA, the basepairs in the internal loop appear to be stabilized, although the RNA chemical shifts indicate that the RNA conformation undergoes some changes when bound by Rev peptide.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 07:41:39