Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ALTERNATIVE SPLICING OF CD44 PRE-MESSENGER-RNA IN HUMAN COLORECTAL TUMORS
Autore:
FINN L; DOUGHERTY G; FINLEY G; MEISLER A; BECICH M; COOPER DL;
Indirizzi:
UNIV PITTSBURGH,SCH MED,DEPT PATHOL PITTSBURGH PA 15261 UNIV PITTSBURGH,SCH MED,DEPT PATHOL PITTSBURGH PA 15261 VET ADM MED CTR,DEPT MED PITTSBURGH PA 15261 BRITISH COLUMBIA CANC AGCY,TERRY FOX LAB VANCOUVER V5Z 1L3 BC CANADA
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 2, volume: 200, anno: 1994,
pagine: 1015 - 1022
SICI:
0006-291X(1994)200:2<1015:ASOCPI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOCYTE HOMING RECEPTOR; HIGH ENDOTHELIUM; CELLS; EXONS; GENE; DNA; RNA; SPERMATOGENESIS; PROTEOGLYCAN; POLYMERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
L. Finn et al., "ALTERNATIVE SPLICING OF CD44 PRE-MESSENGER-RNA IN HUMAN COLORECTAL TUMORS", Biochemical and biophysical research communications, 200(2), 1994, pp. 1015-1022

Abstract

Expression of the CD44 molecule has been linked to tumor growth and metastases in both human and rodent cancers. Alternatively spliced variants expressed in rat and mouse tumors have been shown to confer metastatic potential to non-metastatic carcinoma cell lines, and human homologues of rat variant mRNA sequences are expressed in human tumors. Inthe present study matched sets of RNA from adenocarcinomas of the colon and distant normal mucosa were assayed for CD44 expression by quantitative RT-PCR. Retrospective analysis revealed that colonic tumor cells had both quantitative and qualitative differences in CD44 expression when compared to normal mucosa. These were: 1) an increase in levelsof CD44 transcripts, 2) an increase in levels of alternatively spliced transcripts, 3) the presence of larger alternatively spliced transcripts with inserts >400 bases and 4) the primary alternatively spliced CD44 isoform in colonic adenocarcinomas in all cases is CD44R. Interestingly, two patterns of CD44 isoform expression termed ''variant dominant'' or ''balanced'' patterns of expression, based on the ratio of variant to standard CD44 transcripts (R+V's/H),could be differentiated. An unfavorable prognosis was suggested for tumors expressing increasedlevels of CD44 variant exons previously associated with tumor metastasis. Specifically, patients with tumors expressing the ''variant dominant'' pattern of expression irregardless of Dukes classification and Dukes C and D staged tumors of both patterns exhibited a poorer prognosis. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:33:18