Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECTS OF PARTICULATE MATERIALS AND OSMOPROTECTANTS ON VERY-HIGH-GRAVITY ETHANOLIC FERMENTATION BY SACCHAROMYCES-CEREVISIAE
Autore:
THOMAS KC; HYNES SH; INGLEDEW WM;
Indirizzi:
UNIV SASKATCHEWAN,DEPT APPL MICROBIOL & FOOD SCI SASKATOON S7N 0W0 SKCANADA
Titolo Testata:
Applied and environmental microbiology
fascicolo: 5, volume: 60, anno: 1994,
pagine: 1519 - 1524
SICI:
0099-2240(1994)60:5<1519:EOPMAO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
WATER RELATIONS; GLYCEROL PRODUCTION; COMPATIBLE SOLUTES; ALCOHOL PRODUCTION; WHEAT MASHES; STRESS; OSMOREGULATION; VIABILITY; YEASTS; ACCUMULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
K.C. Thomas et al., "EFFECTS OF PARTICULATE MATERIALS AND OSMOPROTECTANTS ON VERY-HIGH-GRAVITY ETHANOLIC FERMENTATION BY SACCHAROMYCES-CEREVISIAE", Applied and environmental microbiology, 60(5), 1994, pp. 1519-1524

Abstract

The effects of osmoprotectants (such as glycine betaine and proline) and particulate materials on the fermentation of very high concentrations of glucose by the brewing strain Saccharomyces cerevisiae (uvarum)NCYC 1324 were studied. The yeast growing at 20 degrees C consumed only 15 g of the sugar per 100 ml from a minimal medium which initially contained 35% (wt/vol) glucose. Supplementing the medium with a mixture of glycine betaine, glycine, and proline increased the amount of sugar fermented to 30.5 g/100 ml. With such supplementation, the viability of the yeast cells was maintained above 80% throughout the fermentation, while it dropped to less than 12% in the unsupplemented controls. Among single additives, glycine was more effective than proline or glycine betaine. On incubating the cultures for 10 days, the viability decreased to only 55% with glycine, while it dropped to 36 and 27%, respectively, with glycine betaine and proline. It is suggested that glycine and proline, known to be poor nitrogen sources for growth, may serve directly or indirectly as osmoprotectants. Nutrients such as tryptone, yeast extract, and a mixture of purine and pyrimidine bases increased the sugar uptake and ethanol production but aid not allow the population to maintain the high level of cell viability. While only 43% ofthe sugar was fermented in unsupplemented medium, the presence of particulate materials such as wheat bran, wheat mash insolubles, alumina,and soy flour increased sugar utilization to 68, 75, 81, and 82%, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 10:01:16