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Titolo:
STRUCTURE AND REGULATION OF THE CHICKEN ERYTHROID DELTA-AMINOLEVULINATE SYNTHASE GENE
Autore:
LIM KC; ISHIHARA H; RIDDLE RD; YANG ZY; ANDREWS N; YAMAMOTO M; ENGEL JD;
Indirizzi:
NORTHWESTERN UNIV,DEPT BIOCHEM MOLEC BIOL & CELL BIOL,2153 SHERIDAN RD EVANSTON IL 60208 NORTHWESTERN UNIV,DEPT BIOCHEM MOLEC BIOL & CELL BIOL EVANSTON IL 60208 TOHOKU UNIV,SCH MED,DEPT BIOCHEM SENDAI JAPAN HARVARD UNIV,SCH MED,DIV HEMATOL ONCOL BOSTON MA 02115 HARVARD UNIV,SCH MED,DEPT GENET BOSTON MA 02115
Titolo Testata:
Nucleic acids research
fascicolo: 7, volume: 22, anno: 1994,
pagine: 1226 - 1233
SICI:
0305-1048(1994)22:7<1226:SAROTC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
IRON-RESPONSIVE ELEMENT; BETA-GLOBIN GENE; 5-AMINOLEVULINATE SYNTHASE; MESSENGER-RNA; NUCLEOTIDE-SEQUENCE; HEMATOPOIETIC-CELLS; OPTIC TECTUM; EXPRESSION; TRANSCRIPTION; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
K.C. Lim et al., "STRUCTURE AND REGULATION OF THE CHICKEN ERYTHROID DELTA-AMINOLEVULINATE SYNTHASE GENE", Nucleic acids research, 22(7), 1994, pp. 1226-1233

Abstract

Erythroid cells regulate heme biosynthesis in a manner that is distinct from all other cell types. While heme negatively regulates the synthesis of the housekeeping delta-aminolevulinate synthase (ALAS-N) in all non-erythroid cells, the expression of an erythroid-specific isozyme (ALAS-E) is developmentally regulated in red blood cells. As a firststep towards understanding the molecular basis for the transcriptional regulation of ALAS-E during erythropoiesis, we cloned and characterized the chicken ALAS-E locus. This gene spans 18 kbp and is composed of eleven exons. The intron/exon structure of erythroid ALAS was found to be conserved among several vertebrate species. Direct RNA sequencing identified a 5' untranslated region that is derived from two contiguous exons and is predicted to form a very stable stem-loop structure that bears resemblance to the ferritin iron-responsive element. Tissue-specific expression of the ALAS-E gene was analyzed by transient transfection assays in hematopoietic cells of both erythroid and non-erythroid origins. These experiments identified distal (- 784 to - 505 bp) and proximal (- 155 to + 21 bp) promoter elements which are required for high level, erythroid-specific transcription.

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Documento generato il 25/11/20 alle ore 07:21:22