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Titolo:
A CLUSTER OF ESTERASE GENES ON CHROMOSOME 3R OF DROSOPHILA-MELANOGASTER INCLUDES HOMOLOGS OF ESTERASE GENES CONFERRING INSECTICIDE RESISTANCE IN LUCILIA-CUPRINA
Autore:
SPACKMAN ME; OAKESHOTT JG; SMYTH KA; MEDVECZKY KM; RUSSELL RJ;
Indirizzi:
CSIRO,DIV ENTOMOL,GPO BOX 1700 CANBERRA ACT 2601 AUSTRALIA CSIRO,DIV ENTOMOL CANBERRA ACT 2601 AUSTRALIA AUSTRALIAN NATL UNIV,FAC SCI,DIV BIOCHEM & MOLEC BIOL CANBERRA ACT 2601 AUSTRALIA
Titolo Testata:
Biochemical genetics
fascicolo: 1-2, volume: 32, anno: 1994,
pagine: 39 - 62
SICI:
0006-2928(1994)32:1-2<39:ACOEGO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
JUVENILE-HORMONE ESTERASE; MALATHION RESISTANCE; CALLIPHORIDAE; DIPTERA; LOCUS; AMPLIFICATION; MOSQUITOS; VIRILIS; MOTH;
Keywords:
ESTERASES; DROSOPHILA MELANOGASTER; ORGANOPHOSPHATE RESISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
M.E. Spackman et al., "A CLUSTER OF ESTERASE GENES ON CHROMOSOME 3R OF DROSOPHILA-MELANOGASTER INCLUDES HOMOLOGS OF ESTERASE GENES CONFERRING INSECTICIDE RESISTANCE IN LUCILIA-CUPRINA", Biochemical genetics, 32(1-2), 1994, pp. 39-62

Abstract

We identify an esterase isozyme in Drosophila melanogaster, EST 23, which shares biochemical, physiological, and genetic properties with esterase E3, which is involved in resistance to organophosphate insecticides in Lucilia cuprina. Like E3, the D. melanogaster EST 23 is a membrane-bound alpha-esterase which migrates slowly toward the anode at pH6.8. Both enzymes have similar preferences for substrates with shorter acid side chain lengths. Furthermore, on the basis of their high sensitivity to inhibition by paraoxon and their insensitivity to inhibition by eserine sulfate, both enzymes were classified as subclass I carboxylesterases. The activity of each enzyme peaks early in development and, again, in the adult stage. Both enzymes are found in the male reproductive system and larval and adult digestive tissues, the latter being consistent with a role for these enzymes in organophosphate resistance. Fine structure deficiency mapping localized Est 23 to cytological region 84D3 to E1-2 on the right arm of chromosome 3. Moreover, we show that the genes encoding three other esterase phenotypes also map to the same region; these phenotypes involve allozymic differences in EST 9 (formerly EST C), ali-esterase activity, defined by the hydrolysis of methyl butyrate, and malathion carboxylesterase activity, definedby hydrolysis of the organophosphate malathion. This cluster corresponds closely to that encompassing E3 and malathion carboxylesterase on chromosome 4 in L. cuprina, the homologue of chromosome 3R in D. melanogaster.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:12:00