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Titolo:
EPSTEIN-BARR-VIRUS (EBV) REPLICATIVE GENE-EXPRESSION IN TUMOR-CELLS OF AIDS-RELATED NON-HODGKINS-LYMPHOMA IN RELATION TO CD4 CELL NUMBER AND ANTIBODY-TITERS TO EBV
Autore:
BROUSSET P; DROUET E; SCHLAIFER D; ICART J; PAYEN C; MEGGETTO F; MARCHOU B; MASSIP P; DELSOL G;
Indirizzi:
CHU PURPAN,DEPT PATHOL,ANAT PATHOL LAB,PL DOCTEUR BAYLAC F-31059 TOULOUSE FRANCE CHU PURPAN,CIGH,CNRS TOULOUSE FRANCE INST PASTEUR,DEPT VIROL LYON FRANCE CHU PURPAN,DEPT HAEMATOL TOULOUSE FRANCE CHU RANGUEIL,DEPT VIROL RANGUEIL FRANCE CHU PURPAN,DEPT INFECT DIS TOULOUSE FRANCE
Titolo Testata:
AIDS
fascicolo: 5, volume: 8, anno: 1994,
pagine: 583 - 590
SICI:
0269-9370(1994)8:5<583:E(RGIT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; REED-STERNBERG CELLS; TRANSACTIVATOR ZEBRA ANTIBODIES; INSITU HYBRIDIZATION; B-CELL; NASOPHARYNGEAL CARCINOMA; MEMBRANE-PROTEIN; HOMOSEXUAL MEN; HIV-INFECTION; DISEASE;
Keywords:
AIDS; LYMPHOMA; EPSTEIN-BARR VIRUS; REPLICATIVE GENES; SEROLOGY; ZEBRA PROTEIN; CD4 CELL COUNT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
P. Brousset et al., "EPSTEIN-BARR-VIRUS (EBV) REPLICATIVE GENE-EXPRESSION IN TUMOR-CELLS OF AIDS-RELATED NON-HODGKINS-LYMPHOMA IN RELATION TO CD4 CELL NUMBER AND ANTIBODY-TITERS TO EBV", AIDS, 8(5), 1994, pp. 583-590

Abstract

Objective: To determine whether activation of Epstein-Barr virus (EBV) replication in tumour cells of AIDS-related non-Hodgkin's lymphoma (ARNHL) is correlated with CD4+ cell counts and influences antibody response to EBV [anti-Z Epstein-Barr replicative activator (ZEBRA), anti-early antigen (EA), anti-viral capsid antigen (VCA)]. Design: Retrospective study based on immunohistochemistry and in situ hybridization todetect EBV replicative gene products in tissue samples from patients affected by ARNHL and correlation with CD4+ cell counts and results ofEBV serology (including anti-ZEBRA activity) in sera from the same patients. Methods: Seventeen out of 22 cases of ARNHL were selected for the presence of EBV [Epstein-Barr early region (EBER) RNA-positive]. Immunohistochemistry was performed with anti-ZEBRA, anti-EA-restricted,anti-VCA antibodies and in situ hybridization with BHLF1/Notl oligoprobes on tumour samples. Results were statistically correlated with those of CD4+ cell counts (17 out of 17) and with anti-EBV antibody titres (13 out of 17) assessed using standard immunofluorescence method andenzyme-linked immunosorbent assay procedure using recombinant ZEBRA protein and synthetic peptides as antigens. Results: BZLF1 (ZEBRA) or early gene products (EA-R and EA-D/BHLF1/Notl) were detected in a smallproportion (<0.01-5%) of tumour cells in eight of these 17 cases by immunohistochemistry and in situ hybridization. Demonstration of replicative gene expression did not correlate with either low CD4+ cell counts (P>0.05) or anti-EBV antibody titres (P>0.05). Anti-ZEBRA activity was not significantly increased in patients affected with ARNHL, the cells of which expressed replicative gene products (P>0.05). Conclusion: The degree of immunodeficiency does not clearly enhance replicative gene expression in tumour cells of ARNHL. EBV serology, including anti-ZEBRA activity, is not a reliable tool for predicting the occurrence of such proliferations.

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Documento generato il 20/09/20 alle ore 05:01:04