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Titolo:
IDENTIFICATION OF A PROTEIN ENCODED BY A MOUSE SIMPLE REPEATED SEQUENCE
Autore:
DICARLO M; ROMANCINO DP; GHERSI G; MONTANA G; MONTELEONE D;
Indirizzi:
CNR,IST BIOL SVILUPPO,VIA ARCHIRAFI 20 I-90123 PALERMO ITALY CNR,INST DEV BIOL PALERMO ITALY DEPT CELLULAR & DEV BIOL PALERMO ITALY
Titolo Testata:
Journal of submicroscopic cytology and pathology
fascicolo: 2, volume: 26, anno: 1994,
pagine: 255 - 262
SICI:
1122-9497(1994)26:2<255:IOAPEB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPETITIVE DNA-SEQUENCES; EMBRYONIC-DEVELOPMENT; MESSENGER-RNA; GLOBIN GENES; EXPRESSION; FAMILY; GENOME; B1; COMPLEMENTARY; ELEMENTS;
Keywords:
FUSION PROTEIN; ORF; POLYCLONAL ANTIBODIES; SIMPLE REPEATED SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
M. Dicarlo et al., "IDENTIFICATION OF A PROTEIN ENCODED BY A MOUSE SIMPLE REPEATED SEQUENCE", Journal of submicroscopic cytology and pathology, 26(2), 1994, pp. 255-262

Abstract

In order to study the possibility that a mouse repeated 'simple sequence', containing an ORF, could encode a protein, we inserted a fragment of a cDNA clone into the expression vector pEX31C. The fragment containing the short sequence 'CAGAGAGG' was expressed as MS2 polymerase fusion protein in Escherichia coli. This fusion protein (H5fp) was injected subcutaneously into rabbit and the corresponding polyclonal antibodies generated. Western blot analysis of proteins extracted from different mouse tissues established that anti H5fp antibodies recognized, in vivo, an antigen of 28 KDa. Immunolocalization with anti H5fp antibodies showed the presence of the related antigen in the cytoplasm of the examined sections. An hypothetical secondary structure for the protein was predicted by the Chou and Fasman method.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 19:55:18