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Titolo:
EFFECTS OF NH4CL AND NOCODAZOLE ON POLARIZED FIBRONECTIN SECRETION VARY AMONGST DIFFERENT EPITHELIAL-CELL TYPES
Autore:
LOW SH; WONG SH; TANG BL; HONG WJ;
Indirizzi:
NATL UNIV SINGAPORE,INST MOLEC & CELL BIOL,MEMBRANE BIOL LAB,10 KENT RIDGE CRESCENT SINGAPORE 0511 SINGAPORE NATL UNIV SINGAPORE,INST MOLEC & CELL BIOL,MEMBRANE BIOL LAB SINGAPORE 0511 SINGAPORE
Titolo Testata:
Molecular membrane biology
fascicolo: 1, volume: 11, anno: 1994,
pagine: 45 - 54
SICI:
0968-7688(1994)11:1<45:EONANO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANINE KIDNEY-CELLS; PLASMA-MEMBRANE PROTEINS; DIPEPTIDYL PEPTIDASE-IV; EPIDERMAL GROWTH-FACTOR; BRUSH-BORDER MEMBRANE; MDCK CELLS; BIOGENETIC PATHWAYS; SURFACE POLARITY; MAMMALIAN-CELLS; FACTOR RECEPTOR;
Keywords:
EPITHELIAL CELLS; FIBRONECTIN SECRETION; NOCODAZOLE; AMMONIUM CHLORIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
S.H. Low et al., "EFFECTS OF NH4CL AND NOCODAZOLE ON POLARIZED FIBRONECTIN SECRETION VARY AMONGST DIFFERENT EPITHELIAL-CELL TYPES", Molecular membrane biology, 11(1), 1994, pp. 45-54

Abstract

The extracellular matrix protein fibronectin was found to be secretedby three polarized epithelial cell lines Madin-Darby canine kidney (MDCK), Caco-2 and LLC-PK1. About 54 and 46% of fibronectin was secretedfrom the apical and basolateral cell surfaces, respectively, in MDCK cells. in Caco-2 and LLC-PK1 cells, the majority (about 92-93%) of fibronectin secretion occurs from the basolateral cell surface, with the remaining 7-8% from the apical surface. In all three cell types, NH4Clwas found to inhibit basolateral secretion (resulting in enhanced apical secretion), white total fibronection secretion was not significantly affected (although a delay in secretion was observed). Nocodazole reduced total fibronectin secretion to about 70% of control levels in MDCK and Caco-2 cells, with significant inhibition on secretion from both surfaces. In contrast, total fibronectin secretion was enhanced by nocodazole in LLC-PK1 cells. Furthermore, the majority of fibronectin secretion was redirected to the apical cell surface in LLC-PK1 cells. These observations demonstrate that the nature as well as the extent of the effects of NH4Cl and nocodazole on polarized fibronectin secretion varies amongst different epithelial cell types.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:44:23