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Titolo:
UPTAKE OF CD-METALLOTHIONEIN INTO LLC-PK1 CELLS - A COMPARATIVE-STUDYWITH CDCL2
Autore:
KIMURA O; ENDO T; SAKATA M;
Indirizzi:
HLTH SCI UNIV HOKKAIDO,FAC PHARMACEUT SCI,1757 ISHIKARI TOBETSU ISHIKARI HOKKAIDO 06102 JAPAN HLTH SCI UNIV HOKKAIDO,FAC PHARMACEUT SCI ISHIKARI HOKKAIDO 06102 JAPAN
Titolo Testata:
Biological & pharmaceutical bulletin
fascicolo: 2, volume: 20, anno: 1997,
pagine: 158 - 162
SICI:
0918-6158(1997)20:2<158:UOCILC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CADMIUM-METALLOTHIONEIN; BASOLATERAL UPTAKE; PROXIMAL TUBULES; CYSTEINE; BINDING; KIDNEY; NEPHROTOXICITY; NEPHROPATHY; GLUTATHIONE; FORMS;
Keywords:
CADMIUM; METALLOTHIONEIN; LLC-PK1 CELL; UPTAKE; METABOLIC INHIBITOR; PH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
O. Kimura et al., "UPTAKE OF CD-METALLOTHIONEIN INTO LLC-PK1 CELLS - A COMPARATIVE-STUDYWITH CDCL2", Biological & pharmaceutical bulletin, 20(2), 1997, pp. 158-162

Abstract

The uptake of the Cd-metallothionein complex (CdMT) into LLC-PK1 cells was investigated and compared with that of CdCl2. The cells were incubated at 37 degrees C for up to 45 min with 1 mu M Cd, as either CdMTor CdCl2 at pH 5.5, 6.4, or 7.4. Under all the experimental conditions described below, the accumulation of Cd from CdMT was markedly lowerthan that from CdCl2. Cd accumulation at pH 7.4 from CdMT increased linearly with the time of incubation, whereas Cd accumulation from CdCl2 was saturable, Metabolic inhibitors, 2,4-dinitrophenol (DNP) and carbonylcyanide p-(trifluoromethoxy)-phenylhydrazone (FCCP), and incubation at low temperature significantly decreased Cd accumulation from both Cd compounds, Coincubation with 30 mu M ZnCl2, an antagonist of CdCl2 uptake, slightly decreased Cd accumulation from CdMT, but it markedly decreased that from CdCl2. Cd accumulation from CdMT at pH 5.5 was significantly higher than at pH 6.4 or 7.4, whereas Cd accumulation from CdCl2 at pH 5.5 and 6.4 was significantly lower than at pH 7.4. Although Cd accumulation from CdCl2 at pH 7.4 was significantly decreased by coincubation with 100 mu M cysteine or glutathione (GSH), this decrease was not observed at pH 5.5 or 6.4, A small amount of Cd was removed, by the chelating agent EGTA, from the cell membranes after incubation with CdMT at pH 6.4 and 7.4, whereas a considerable amount of Cd was removed by EGTA after incubation with CdMT at pH 5.5 and with CdCl2at three pHs. It appears that the CdMT complex is taken up into LLC-PK1 cells partially ria an energy-dependent process, and the increase in Cd accumulation at low pH is due to the liberation of Cd, High stability and molecular size of the CdMT complex explains why it is not taken up readily into LLC-PK1 cells.

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Documento generato il 02/12/20 alle ore 15:07:17