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Titolo:
IN-VITRO EVOLUTION OF A NEUTRALIZING HUMAN-ANTIBODY TO HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TO ENHANCE AFFINITY AND BROADEN STRAIN CROSS-REACTIVITY
Autore:
BARBAS CF; HU D; DUNLOP N; SAWYER L; CABABA D; HENDRY RM; NARA PL; BURTON DR;
Indirizzi:
SCRIPPS CLIN & RES INST,DEPT MOLEC BIOL,10666 N TORREY PINES RD LA JOLLA CA 92037 SCRIPPS CLIN & RES INST,DEPT IMMUNOL LA JOLLA CA 92037 NCI,FREDERICK CANC RES & DEV CTR,VIRUS BIOL SECT,TUMOR CELL BIOL LAB FREDERICK MD 21702 CALIF DEPT HLTH SERV,VIRAL & RICKETTSIAL DIS LAB BERKELEY CA 94704
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 9, volume: 91, anno: 1994,
pagine: 3809 - 3813
SICI:
0027-8424(1994)91:9<3809:IEOANH>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MONOCLONAL-ANTIBODY; SOLUBLE CD4; GP120; INFECTION; PREVENTION; IMMUNIZATION; CHIMPANZEES; RETROVIRUS; LIBRARIES; RECEPTOR;
Keywords:
PASSIVE IMMUNIZATION; VIRAL NEUTRALIZATION; PHAGE DISPLAY; COMBINATORIAL LIBRARIES; COMPLEMENTARITY-DETERMINING REGIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
C.F. Barbas et al., "IN-VITRO EVOLUTION OF A NEUTRALIZING HUMAN-ANTIBODY TO HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TO ENHANCE AFFINITY AND BROADEN STRAIN CROSS-REACTIVITY", Proceedings of the National Academy of Sciences of the United Statesof America, 91(9), 1994, pp. 3809-3813

Abstract

A method is described that allows for the improvement of antibody affinity. This method, termed complementary-determining region (CDR) walking, does not require structural information on either antibody or antigen. Complementary-determining regions are targeted for random mutagenesis followed by selection for fitness, in this case increased binding affinity, by the phage-display approach. The current study targets ahuman CD4-binding-site anti-gp120 antibody that is potently and broadly neutralizing. Evolution of affinity of this antibody demonstrates in this case that affinity can be increased while reactivity to variants of human immunodeficiency virus type 1 is broadened. The neutralizing ability of this antibody is improved, as assayed with laboratory andprimary clinical isolates of human inmunodeficiency virus type 1. Theability to produce human antibodies of exceptional affinity and broadneutralizing ability has implications for the therapeutic and prophylactic application of antibodies for human immunodeficiency virus type 1 infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:44:56