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Titolo:
REVERSAL OF METHOTREXATE-INDUCED FOLATE POOL DEPLETION BY THYMIDINE IN A HUMAN LEUKEMIA-CELL LINE IN-VITRO
Autore:
SUR P; MATSUO Y; OTANI T; MINOWADA J;
Indirizzi:
HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR,675-1 FUJISAKI OKAYAMA702 JAPAN HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR OKAYAMA 702 JAPAN
Titolo Testata:
Tumori
fascicolo: 6, volume: 79, anno: 1993,
pagine: 433 - 438
SICI:
0300-8916(1993)79:6<433:ROMFPD>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFERENTIATION; HL-60; MODULATION; INTERFERON; INDUCTION; AGENTS;
Keywords:
METHOTREXATE; INTERFERON-GAMMA; THYMIDINE; FOLATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
P. Sur et al., "REVERSAL OF METHOTREXATE-INDUCED FOLATE POOL DEPLETION BY THYMIDINE IN A HUMAN LEUKEMIA-CELL LINE IN-VITRO", Tumori, 79(6), 1993, pp. 433-438

Abstract

In an in vitro study using a human monocytic leukemia cell line, U-937, the effects of interferon-gamma (IFN-gamma) in combination with theantifolate methotrexate and the role of thymidine introduced as a biochemical modulator were investigated. Methotrexate alone or in combination with INF-gamma was found to enhance the induction of morphologic and functional monocytic differentiation in the U-937 cell line. Various cellular effects with the addition of thymidine to the medium with methotrexate and IFN-gamma were studied. Enhanced inhibition of cell growth and perturbation of the cell cycle were noted when methotrexate and IFN-gamma were used in combination, but not when methotrexate was used alone. The reduction of cellular folate by methotrexate was also enhanced in combination with IFN-gamma. Cell cycle delay, resulting incell growth inhibition of folate depletion, caused the Induction of differentiation in U-937 cells, which was found to be greater with methotrexate + IFN-gamma than with methotrexate alone. Cellular differentiation, as assessed by nitroblue tetrazolium reduction assay, indirect immunofluorescence and morphology, showed better effects towards the differentiation of U-937 cells when the agents were used in combination. However, addition of thymidine to the medium was found to cancel allthe aforementioned effects. The addition of thymidine to the medium also caused reversal of the inhibitory effect of methotrexate and IFN-gamma on cell growth and repletion of the endogenous folate level. Repletion of the folate level by exogenous thymidine is a new possibility for the role of the thymidine in cellular growth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 15:11:41