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Titolo:
POLYMERASE CHAIN-REACTION ASSAY FOR DETECTION OF SHEEP AND GOAT MEATS
Autore:
CHIKUNI K; TABATA T; KOSUGIYAMA M; MONMA M; SAITO M;
Indirizzi:
NATL FOOD RES INST TSUKUBA IBARAKI 305 JAPAN IBARAKI UNIV AMI IBARAKI 30003 JAPAN
Titolo Testata:
Meat science
fascicolo: 3, volume: 37, anno: 1994,
pagine: 337 - 345
SICI:
0309-1740(1994)37:3<337:PCAFDO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA HYBRIDIZATION; ENZYMATIC AMPLIFICATION; DIFFERENTIATION; SEQUENCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
K. Chikuni et al., "POLYMERASE CHAIN-REACTION ASSAY FOR DETECTION OF SHEEP AND GOAT MEATS", Meat science, 37(3), 1994, pp. 337-345

Abstract

Polymerase Chain Reaction (PCR) was applied to a qualitative differentiation between sheep, goat and bovine meats. Oligonucleotide primers were designed for the amplification of sheep satellite I DNA sequence. The PCR amplified 374 bp fragments from sheep and goat DNA, but no fragment from bovine, water buffalo, sika deer, pig, horse, rabbit and chicken DNA. Sheep DNA (10 pg) was detected by 4% agarose gel electrophoresis following PCR amplification. Although cooking of the sample meats reduced the PCR products, sheep DNA was detected in the meat heatedat 120-degrees-C In order to differentiate between sheep and goat meats, nucleotide sequences of the PCR products were determined directly by cycle sequencing. The sequence of PCR products showed 920% of homology between sheep and goat. They were differentiated by ApaI digestionof the PCR products because sheep had one ApaI site and goat had no site in the PCR products.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 00:40:51