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Titolo:
QUANTIFICATION OF MICROTUBULE DYNAMICS IN LIVING PLANT-CELLS USING FLUORESCENCE REDISTRIBUTION AFTER PHOTOBLEACHING
Autore:
HUSH JM; WADSWORTH P; CALLAHAM DA; HEPLER PK;
Indirizzi:
UNIV SYDNEY,SCH BIOL SCI SYDNEY NSW 2006 AUSTRALIA
Titolo Testata:
Journal of Cell Science
, volume: 107, anno: 1994,
parte:, 4
pagine: 775 - 784
SICI:
0021-9533(1994)107:<775:QOMDIL>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOTIC SPINDLES; CORTICAL MICROTUBULES; LABELED TUBULIN; SEA-URCHIN; INVITRO; POLARITY; KINASE; POLYMERIZATION; REORIENTATION; MICROSCOPY;
Keywords:
MICROTUBULE; TRADESCANTIA; FRAP; CELL DIVISION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Hush et al., "QUANTIFICATION OF MICROTUBULE DYNAMICS IN LIVING PLANT-CELLS USING FLUORESCENCE REDISTRIBUTION AFTER PHOTOBLEACHING", Journal of Cell Science, 107, 1994, pp. 775-784

Abstract

Microtubule (MT) turnover within the four principal MT arrays, the cortical array, the preprophase band, the mitotic spindle and the phragmoplast, has been measured in living stamen hair cells of Tradescantia.that have been injected with fluorescent neurotubulin. Using the combined techniques of confocal laser scanning microscopy and fluorescenceredistribution after photobleaching (FRAP), we report that the half-time of turnover in spindle MTs is t(1/2)=31+/-6 seconds, which is in excellent agreement with previous measurements of turnover in animal cell spindles. Tradescantia interphase MTs, however, exhibit turnover rates (t(1/2)=67+/-seconds) that are some 3.4-fold faster than those measured in interphase mammalian cells, and thus are revealed as being highly dynamic. Preprophase band and phragmoplast MTs have turnover rates similar to those of interphase MTs in Tradescantia. The spatial and temporal aspects of the fluorescence redistribution after photobleaching in all four MT arrays are more consistent with subunit exchange by the mechanism of dynamic instability than treadmilling. This is the first quantification of MT dynamics in plant cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 22:29:05