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Titolo:
CHARACTERIZATION AND KINETIC-STUDIES OF A NOVEL DYE PREPARED FROM THEOXIDATION OF A WATER-SOLUBLE IMETHYLFERROCENE-2-HYDROXYPROPYL-BETA-CYCLODEXTRIN COMPLEX USING IMMOBILIZED BILIRUBIN OXIDASE
Autore:
MALE KB; LUONG JHT;
Indirizzi:
NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,6100 ROYALMOUNT AVE MONTREAL H4P 2R2 PQ CANADA NATL RES COUNCIL CANADA,BIOTECHNOL RES INST MONTREAL H4P 2R2 PQ CANADA
Titolo Testata:
Enzyme and microbial technology
fascicolo: 5, volume: 16, anno: 1994,
pagine: 425 - 431
SICI:
0141-0229(1994)16:5<425:CAKOAN>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENZYME;
Keywords:
BILIRUBIN OXIDASE; ENZYMATIC OXIDATION; 1,1'-DIMETHYLFERROCENE; 1,1'-DIMETHYLFERRICINIUM; 2-HYDROXYPROPYL-BETA-CYCLODEXTRIN; INCLUSION COMPLEX; ENZYME ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
K.B. Male e J.H.T. Luong, "CHARACTERIZATION AND KINETIC-STUDIES OF A NOVEL DYE PREPARED FROM THEOXIDATION OF A WATER-SOLUBLE IMETHYLFERROCENE-2-HYDROXYPROPYL-BETA-CYCLODEXTRIN COMPLEX USING IMMOBILIZED BILIRUBIN OXIDASE", Enzyme and microbial technology, 16(5), 1994, pp. 425-431

Abstract

Bilirubin oxidase, a commercially available copper containing enzyme isolated from Myrothecium verrucaria, was observed to oxidize a yellowimethylferrocene-2-hydroxypropyl-beta-cyclodextrin inclusion complex to a stable blue dye known as 1,1'-dimethylferricinium at pH 5-7. During the oxidation process, there was a noticeable increase in pH as a result of the consumption of both H+ and oxygen, and the molar ratio between 1,1'-dimethylferrocene and oxygen was established as 4:1. The enzyme was covalently immobilized onto either aminopropyl or arylamine glass beads to form an immobilized enzyme reactor which could be used for the repeated preparation of the blue dye. For bilirubin oxidase immobilized onto the aminopropyl beads, the reaction was much more efficient and was completed within 60-90 min, corresponding to a conversion yield of 84%. The blue dye was reduced instantaneously by ascorbic acid or uric acid to its original reduced form and was insensitive to a wide pH variation from 2 to 11. Application of the blue dye as a colorimetric assay reagent for glucose using beta-D-glucose oxidase, hypoxanthine using xanthine oxidase, and lactate using lactate oxidase was successfully demonstrated. Kinetic studies revealed that the Michaelis-Menten constant for the blue dye with respect to glucose oxidase was noticeably higher than that of lactate oxidase or xanthine oxidase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:45:00