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Titolo:
EXPRESSION OF A BOVINE VESICULAR MONOAMINE TRANSPORTER IN COS CELLS
Autore:
GASNIER B; KREJCI E; BOTTON D; MASSOULIE J; HENRY JP;
Indirizzi:
INST BIOL PHYSICOCHIM,SERV NEUROBIOL PHYSICOCHIM,CNRS,UA 1112,13 RUE PIERRE & MARIE CURIE F-75005 PARIS FRANCE INST BIOL PHYSICOCHIM,SERV NEUROBIOL PHYSICOCHIM,CNRS,UA 1112 F-75005PARIS FRANCE ECOLE NORMALE SUPER,NEUROBIOL LAB,CNRS,UA 295 F-75005 PARIS FRANCE
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 342, anno: 1994,
pagine: 225 - 229
SICI:
0014-5793(1994)342:3<225:EOABVM>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHROMAFFIN GRANULE MEMBRANES; SECRETORY VESICLES; STORAGE-VESICLES; ACCUMULATION; BINDING; CARRIER; GHOSTS;
Keywords:
CHROMAFFIN GRANULE; NEUROTRANSMITTER TRANSPORTER; COS CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
B. Gasnier et al., "EXPRESSION OF A BOVINE VESICULAR MONOAMINE TRANSPORTER IN COS CELLS", FEBS letters, 342(3), 1994, pp. 225-229

Abstract

Catecholamines are accumulated in vesicles by a proton gradient-dependent transport, which has mostly been studied in bovine chromaffin granules. The full sequence of a cDNA encoding a vesicular transporter from bovine chromaffin cells, bVMAT(2), was recently reported. We now present an analysis of bVMAT(2), expressed in transfected COS cells. Comparing the binding of a labelled ligand, H-3TBZOH, and the rate of uptake, we find a much lower molecular turnover number than in chromaffin granules, probably indicating that a majority of expressed transporters are correctly folded and possess the ligand binding site but cannot actively transport monoamines because they are located in compartments which do not possess a proton gradient. The substrate specificity of uptake and its pharmacological sensitivity to various inhibitors closely resemble those previously observed in chromaffin granules. Theseresults suggest that VMAT(2) is the major transporter in bovine adrenal glands, and raise the question of the significance of the second related transporter, VMAT(1), which is also expressed in this tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 13:19:32