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Titolo:
ENTEROCHROMAFFIN CELLS OF THE DIGESTIVE-SYSTEM - CELLULAR SOURCE OF GUANYLIN, A GUANYLATE CYCLASE-ACTIVATING PEPTIDE
Autore:
CETIN Y; KUHN M; KULAKSIZ H; ADERMANN K; BARGSTEN G; GRUBE D; FORSSMANN WG;
Indirizzi:
HANNOVER MED SCH,DEPT ANAT,KONSTANTY GUTSCHOW STR 8 D-30625 HANNOVER GERMANY LOWER SAXONY INST PEPTIDE RES D-30625 HANNOVER GERMANY
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 8, volume: 91, anno: 1994,
pagine: 2935 - 2939
SICI:
0027-8424(1994)91:8<2935:ECOTD->2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
VAGAL NERVE-STIMULATION; STABLE ENTERO-TOXIN; RAT INTESTINAL-CELLS; ESCHERICHIA-COLI; ENDOCRINE-CELLS; CHROMOGRANIN-A; ENDOGENOUS ACTIVATOR; SUBSTANCE-P; SEROTONIN; BINDING;
Keywords:
GUT HORMONES; ENTERO-ENDOCRINE CELLS; INTESTINE; HEAT-STABLE ENTEROTOXIN RECEPTOR; DIARRHEA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
Y. Cetin et al., "ENTEROCHROMAFFIN CELLS OF THE DIGESTIVE-SYSTEM - CELLULAR SOURCE OF GUANYLIN, A GUANYLATE CYCLASE-ACTIVATING PEPTIDE", Proceedings of the National Academy of Sciences of the United Statesof America, 91(8), 1994, pp. 2935-2939

Abstract

Guanylin, a bioactive peptide, has recently been isolated from the intestine; this peptide activates intestinal guanylate cyclase (i.e., guanylate cyclase C) and thus is potentially involved in the regulation of water/electrolyte transport in the gastrointestinal mucosa. As yet,the cells involved in synthesis, storage, or secretion of guanylin have not been identified by immunocytochemistry. We raised antisera against guanylin and investigated the entire gastrointestinal tract of guinea pigs by light and electron microscopical immunocytochemistry. Extracts of various intestinal segments and plasma analyzed on a Western blot revealed a peptide band corresponding to the molecular mass of guanylin. Localization studies in the entire digestive tract showed that guanylin is exclusively confined to enterochromaffin (EC) cells. Remarkably, most EC cells contacted the gut lumen by cell processes that were highly immunoreactive for guanylin. In addition to the well known secretion in an endocrine fashion, EC cells by circumstantial evidence may release guanylin into the gut lumen to activate guanylate cyclase C that is immediately located on the brush border of adjacent enterocytes. The unique localization of guanylin in EC cells may indicate thatthese cells are involved in the regulation of fluid secretion in the gastrointestinal mucous membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:32:32