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Titolo:
MYASTHENIA-GRAVIS - RESIDUES OF THE ALPHA-SUBUNIT AND GAMMA-SUBUNIT OF MUSCLE ACETYLCHOLINE-RECEPTOR INVOLVED IN FORMATION OF IMMUNODOMINANT CD4(+) EPITOPES
Autore:
MOIOLA L; PROTTI MP; MCCORMICK D; HOWARD JF; CONTITRONCONI BM;
Indirizzi:
UNIV MINNESOTA,COLL BIOL SCI,DEPT BIOCHEM,1479 GORTNER AVE ST PAUL MN55108 UNIV MINNESOTA,COLL BIOL SCI,DEPT BIOCHEM ST PAUL MN 55108 UNIV MINNESOTA,SCH MED,DEPT PHARMACOL MINNEAPOLIS MN 55455 MAYO CLIN & MAYO FDN,DEPT BIOCHEM & MOLEC BIOL,MAYO PROT CORE ROCHESTER MN 55905 UNIV N CAROLINA,DEPT NEUROL CHAPEL HILL NC 27599 HOSP SAN RAFFAELE I-20132 MILAN ITALY
Titolo Testata:
The Journal of immunology
fascicolo: 9, volume: 152, anno: 1994,
pagine: 4686 - 4698
SICI:
0022-1767(1994)152:9<4686:M-ROTA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY COMPLEX; T-CELL CLONES; LYMPHOCYTES-T; HLA-DR; AUTOIMMUNE ENCEPHALOMYELITIS; SYNTHETIC PEPTIDES; TARGET EPITOPES; MHC; IDENTIFICATION; HELPER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
70
Recensione:
Indirizzi per estratti:
Citazione:
L. Moiola et al., "MYASTHENIA-GRAVIS - RESIDUES OF THE ALPHA-SUBUNIT AND GAMMA-SUBUNIT OF MUSCLE ACETYLCHOLINE-RECEPTOR INVOLVED IN FORMATION OF IMMUNODOMINANT CD4(+) EPITOPES", The Journal of immunology, 152(9), 1994, pp. 4686-4698

Abstract

We propagated from myasthenia gravis (MC) patients by stimulation in vitro with synthetic sequences (alpha 48-67, alpha 304-322, gamma 75-94 and gamma 321-340) of the human muscle acetylcholine receptor (AChR), CD4(+) lines against four 20-residue sequence regions of the AChR alpha and gamma subunits that are recognized by Th cells of most MG patients. Most lines secreted IL-2 and not IL-4, suggesting that they comprise Th1 cells. For three lines we verified that, as reported previously, AChR epitopes are presented by DR molecules: their response to therelevant peptide was abolished by anti-DR Abs. The DR molecules presenting AChR epitopes were identified by testing the response of the lines to the relevant peptide, using APC from donors homozygous for the different DR alleles of the line. We tested the lines with single residue-substituted analogues of the epitope sequence. The results of theseexperiments indicated that the lines were polyclonal and recognized overlapping epitopes. Their response was abolished by some substitutions, identifying residues common to all epitopes within a given region, whereas other substitutions reduced but did not obliterate the response, indicating residues included in some but not all epitopes recognized by the line. Comparison of the residues involved in epitope formation for different lines supported the conclusion that within the 20-residue immunodominant regions investigated here, the same sequence segment is involved in formation of epitopes, even in DR-discordant patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:27:19