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Titolo:
USE OF RECOMBINANT ADENOVIRUS TO TRANSFER THE HERPES-SIMPLEX VIRUS THYMIDINE KINASE (HSVTK) GENE TO THORACIC NEOPLASMS - AN EFFECTIVE IN-VITRO DRUG SENSITIZATION SYSTEM
Autore:
SMYTHE WR; HWANG HC; AMIN KM; ECK SL; DAVIDSON BL; WILSON JM; KAISER LR; ALBELDA SM;
Indirizzi:
HOSP UNIV PENN,THORAC ONCOL RES LAB,MALONEY BLDG,ROOM 809,36TH & SPRUCE STS PHILADELPHIA PA 19104 UNIV PENN,MED CTR,DEPT SURG,THORAC SURG SECT PHILADELPHIA PA 19104 UNIV PENN,MED CTR,DEPT MED,PULM CRIT CARE SECT PHILADELPHIA PA 19104 UNIV PENN,THORAC ONCOL RES LAB,DEPT MED,HEMATOL ONCOL SECT PHILADELPHIA PA 19104 UNIV PENN,MED CTR,DEPT MOLEC & CELLULAR ENGN PHILADELPHIA PA 19104 UNIV PENN,MED CTR,INST HUMAN GENE THERAPY PHILADELPHIA PA 19104 UNIV MICHIGAN,MED CTR,DEPT MED,RHEUMATOL SECT ANN ARBOR MI 48109
Titolo Testata:
Cancer research
fascicolo: 8, volume: 54, anno: 1994,
pagine: 2055 - 2059
SICI:
0008-5472(1994)54:8<2055:UORATT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHEMOTHERAPY; RADIOTHERAPY; TUMORS; CELLS;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
W.R. Smythe et al., "USE OF RECOMBINANT ADENOVIRUS TO TRANSFER THE HERPES-SIMPLEX VIRUS THYMIDINE KINASE (HSVTK) GENE TO THORACIC NEOPLASMS - AN EFFECTIVE IN-VITRO DRUG SENSITIZATION SYSTEM", Cancer research, 54(8), 1994, pp. 2055-2059

Abstract

Transfer of the herpes simplex virus thymidine kinase (HSVtk) gene into tumor cells using retroviral vectors followed by administration of ganciclovir provides a potential strategy for the treatment of malignancy. Because of the limitations of using retroviral vectors for clinical application, the feasibility of using a recombinant adenovirus containing HSVtk was examined. Cell lines derived from human malignant mesotheliomas and non-small cell lung cancers infected with a recombinantadenovirus containing HSVtk showed strong expression of HSVtk proteinas determined by immunohistochemical staining. Infection with a recombinant adenovirus containing HSVtk rendered cells sensitive to doses of ganciclovir that were 2-3 logs lower than uninfected cells or those infected with a control virus. A strong ''bystander effect'' was notedin mesothelioma lines; there was no diminution in the efficacy of ganciclovir treatment until the ratio of infected:uninfected cells fell below 1:10. This study thus demonstrates in vitro efficacy of an adenovirus-transduced HSVtk drug sensitization gene therapy system in thoracic malignancies. Recombinant adenovirus transfer of the HSVtk gene followed by ganciclovir may have promise as an in situ treatment for tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:29:04