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Titolo:
EVALUATION OF C-ERBB-2 AMPLIFICATION IN BREAST-CARCINOMA BY DIFFERENTIAL POLYMERASE CHAIN-REACTION
Autore:
GRAMLICH TL; COHEN C; FRITSCH C; DEROSE PB; GANSLER T;
Indirizzi:
EMORY UNIV HOSP,DEPT PATHOL & LAB MED,DIV ANAT PATHOL,1364 CLIFTON RDATLANTA GA 30322 EMORY UNIV,SCH MED,DEPT PATHOL & LAB MED ATLANTA GA 30322
Titolo Testata:
American journal of clinical pathology
fascicolo: 4, volume: 101, anno: 1994,
pagine: 493 - 499
SICI:
0002-9173(1994)101:4<493:EOCAIB>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; SOUTHERN BLOT ANALYSIS; ESTROGEN-RECEPTOR; NEU ONCOGENE; PROGNOSTIC-SIGNIFICANCE; PROTO-ONCOGENE; FLOW-CYTOMETRY; IMMUNOHISTOCHEMICAL EVALUATION; HER-2/NEU AMPLIFICATION; IMAGE-ANALYSIS;
Keywords:
BREAST CARCINOMA; C-ERBB-2; ONCOGENE; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
T.L. Gramlich et al., "EVALUATION OF C-ERBB-2 AMPLIFICATION IN BREAST-CARCINOMA BY DIFFERENTIAL POLYMERASE CHAIN-REACTION", American journal of clinical pathology, 101(4), 1994, pp. 493-499

Abstract

Previous studies have found that amplification and overexpression of the c-erbB-2 oncogene in mammary ductal adenocarcinomas predicts decreased disease-free or overall survival. Information regarding the prognostic and pathogenetic significance of oncogene amplification has beenlimited by difficulty obtaining sufficient quantities of high molecular weight DNA for Southern blot analysis. Differential polymerase chain reaction (PCR) has been suggested as an alternative method for evaluating gene amplification and can be performed using formalin-fixed paraffin-embedded specimens. The authors of this study used differential PCR to detect c-erbB-2 gene amplification and immunohistochemistry to evaluate c-erbB-2 expression. A highly significant degree of concordance (P < .002) between c-erbB-2 amplification and expression was observed. Abnormalities of c-erbB-2 copy number or expression were more common in tumors with higher histologic grade, and trends were noted toward association with other prognostically unfavorable biologic markers, such as reduced progesterone receptor content and DNA aneuploidy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 12:48:35