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Titolo:
DETECTION OF THE MBCR ABL TRANSLOCATION IN CHRONIC MYELOID-LEUKEMIA BY FLUORESCENCE IN-SITU HYBRIDIZATION - COMPARISON WITH CONVENTIONAL CYTOGENETICS AND IMPLICATIONS FOR MINIMAL RESIDUAL DISEASE DETECTION/
Autore:
GARCIAISIDORO M; TABERNERO MD; GARCIA JL; NAJERA ML; HERNANDEZ JM; WIEGANT J; RAAP A; SANMIGUEL J; ORFAO A;
Indirizzi:
HOSP UNIV SALAMANCA,HEMATOL LAB,SERV GEN CITOMETRIA SALAMANCA 37007 SPAIN UNIV SALAMANCA,SERV GEN CITOMETRIA E-37008 SALAMANCA SPAIN UNIV SALAMANCA,DEPT BIOL CELULAR E-37008 SALAMANCA SPAIN HOSP UNIV SALAMANCA,SERV HEMATOL SALAMANCA SPAIN LEIDEN UNIV,SYLVIUS LAB NL-2300 RA LEIDEN NETHERLANDS
Titolo Testata:
Human pathology
fascicolo: 2, volume: 28, anno: 1997,
pagine: 154 - 159
SICI:
0046-8177(1997)28:2<154:DOTMAT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC MYELOGENOUS LEUKEMIA; POLYMERASE CHAIN-REACTION; BONE-MARROW TRANSPLANTATION; CHRONIC MYELOCYTIC-LEUKEMIA; CLUSTER REGION REARRANGEMENT; PH-NEGATIVE CML; BCR-ABL FUSION; PHILADELPHIA-CHROMOSOME; INSITU HYBRIDIZATION; MESSENGER-RNA;
Keywords:
BCR/ABL FUSION; FISH; CML;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
M. Garciaisidoro et al., "DETECTION OF THE MBCR ABL TRANSLOCATION IN CHRONIC MYELOID-LEUKEMIA BY FLUORESCENCE IN-SITU HYBRIDIZATION - COMPARISON WITH CONVENTIONAL CYTOGENETICS AND IMPLICATIONS FOR MINIMAL RESIDUAL DISEASE DETECTION/", Human pathology, 28(2), 1997, pp. 154-159

Abstract

The correlation between the detection of the Philadelphia chromosome by conventional cytogenetics and the identification of Mbcr/abl translocation by fluorescence in situ hybridization (FISH) in both metaphaseand interphase cells is prospectively analyzed in a group of 21 chronic myeloid leukemia (CML) patients. To gain insight into the sensitivity and specificity of the detection of the bcr/abl translocation by FISH, a group of 10 healthy volunteers was also studied. Our results show that for the detection of bcr/abl translocation in CML patients, FISH is more sensitive than conventional cytogenetics because it detects significantly higher proportions of cells carrying the translocation both in metaphase (P < .0002) and interphase nuclei (P < .003). Moreover, in the metaphases of the controls analyzed, no bcr/abl+ chromosome was detected that makes the colocalization of bcr and abl signals in the CML, patients highly specific. Conversely, in control interphase nuclei, a small proportion of cells (ranging between 0% and 3%, mean value of 1.7% +/- 0.9%) displaying colocalization of both signals is usually detected. This limits, at least for the moment, the routine use ofFISH for the detection of minimal residual disease in CML patients atlevels lower than 10(-1). Copyright (C) 1997 by W.B. Saunders Company.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:20:43