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Titolo:
EXPRESSION OF BETA-AMYLOID PRECURSOR PROTEIN IN THE DEVELOPING HUMAN SPINAL-CORD
Autore:
ARAI H; HIGUCHI S; MATSUSHITA S; YUZURIHA T; TROJANOWSKI JQ; LEE VMY;
Indirizzi:
MOTOJIMA GEN HOSP,DEPT NEUROL OTA GUNMA 373 JAPAN KURIHAMA NATL HOSP,NATL INST ALCOHOLISM,DEPT PSYCHIAT YOKOSUKA KANAGAWA 239 JAPAN HOSP UNIV PENN,DEPT PATHOL & LAB MED,DIV ANAT PATHOL PHILADELPHIA PA 19104
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 642, anno: 1994,
pagine: 132 - 136
SICI:
0006-8993(1994)642:1-2<132:EOBPPI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALZHEIMERS-DISEASE; MONOCLONAL-ANTIBODIES; NERVOUS-SYSTEM; RAT-BRAIN; PEPTIDE; CELL; IDENTIFICATION; TISSUES;
Keywords:
BETA-AMYLOID PRECURSOR PROTEIN; HUMAN FETAL SPINAL CORD; DEVELOPMENT; IMMUNOSTAINING; WESTERN BLOT ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
H. Arai et al., "EXPRESSION OF BETA-AMYLOID PRECURSOR PROTEIN IN THE DEVELOPING HUMAN SPINAL-CORD", Brain research, 642(1-2), 1994, pp. 132-136

Abstract

Human fetal spinal cords and other non-neural tissues from cases withgestational age from 6 to 21 weeks were examined with a panel of antibodies to different domains of beta-amyloid precursor proteins (beta-APPs). In the early developmental stages, the beta-APPs were expressed in three distinct layers, i.e., primitive neuroepithelial cell layer, mantle layer and marginal layer. beta-APP immunoreactivity was most prominent in cell bodies of putative neuroblasts located in the outer ventral part of the mantle layer. beta-APP expression diminished as the spinal cord matured and a weak residual immunoreactivity was detected exclusively in a subset of the anterior horn cells by 21 weeks gestational age. Throughout the gestational ages examined, no convincing beta/A4 immunostaining was seen in any of the spinal cord regions. Outsidethe spinal cord, beta-APP immunostaining was consistently present in (1) cell bodies and proximal nerves of immature neurons of dorsal rootganglia and in (2) myotubules, although these cells were devoid of beta/A4 immunoreactivity. Western blot analysis of fetal spinal cord revealed immunoreactive bands with apparant molecular weight between 100 and 140 kDa in the membrane-associated fraction, while soluble proteins with a molecular mass centered on 115 kDa were detected in the cytosolic fraction. Our results indicate that: (1) one or more isoforms of full length beta-APPs are expressed at very early gestational ages in the developing human spinal cord; (2) the normal metabolism of beta-APPs does not result in accumulations of beta/A4 fragments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 16:53:38