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Titolo:
RHODAMINE-PHALLOIDIN AND ANTITUBULIN ANTIBODY STAINING OF SPINDLE FIBERS THAT WERE IRRADIATED WITH AN ULTRAVIOLET MICROBEAM
Autore:
CZABAN BB; FORER A;
Indirizzi:
YORK UNIV,DEPT BIOL,4700 KEELE ST N YORK M3J 1P3 ON CANADA YORK UNIV,DEPT BIOL N YORK M3J 1P3 ON CANADA
Titolo Testata:
Protoplasma
fascicolo: 1-2, volume: 178, anno: 1994,
pagine: 18 - 27
SICI:
0033-183X(1994)178:1-2<18:RAAASO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRANE-FLY SPERMATOCYTES; ROOT-TIP CELLS; F-ACTIN; HIGHER-PLANT; CHROMOSOMAL MOVEMENT; CYTOPLASMIC DYNEIN; MITOTIC SPINDLES; MICROTUBULES; ANAPHASE; FIBERS;
Keywords:
ACTIN FILAMENTS; CHROMOSOMAL SPINDLE FIBERS; CHROMOSOME MOVEMENT; MICROTUBULES; ULTRAVIOLET MICROBEAM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
B.B. Czaban e A. Forer, "RHODAMINE-PHALLOIDIN AND ANTITUBULIN ANTIBODY STAINING OF SPINDLE FIBERS THAT WERE IRRADIATED WITH AN ULTRAVIOLET MICROBEAM", Protoplasma, 178(1-2), 1994, pp. 18-27

Abstract

We irradiated chromosomal spindle fibres in crane-fly spermatocytes with an ultraviolet microbeam of 270 nm wavelength light with total energies near those that cause actin filaments in myofibrils to depolymerize; after irradiation we stained the cells with rhodamine-labelled phalloidin and with anti-tubulin antibodies. In some cells, the irradiation reduced both phalloidin and tubulin staining of the chromosomal spindle fibres; in other cells, the irradiations reduced phalloidin staining but not tubulin staining; in yet other cells, the irradiations reduced tubulin staining but not phalloidin staining. In all irradiated cells in which phalloidin staining was reduced in the irradiated areasphalloidin staining also was reduced poleward from the irradiated areas. These results show that phalloidin staining of chromosomal spindlefibres is not dependent on the presence of kinetochore microtubules, and, therefore, that actin filaments are present in the spindle fibresin vivo. We suggest that actin filaments present in spindle fibres invivo may be involved in causing chromosome movements during anaphase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 06:13:56